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浙江洞头岛自由生活线虫两个优势种的形态描述及其系统发育分析OA北大核心CSTPCD

Morphological descriptions and phylogenetic analysis of two dominant species of free-living marine nematodes in Dongtou Island,Zhejiang

中文摘要英文摘要

为了了解浙江洞头岛自由生活线虫(Nematoda)的种类和多样性,作者对该海域线虫进行分类研究,发现了 2 个优势种并进行了重新描述.其中,丑萨巴线虫(Sabatieria foetida Gagarin&Nguyen Vu Thanh,2008)为一新纪录种,主要特征为:表皮具环状排列的小点,有较大的点组成的侧装饰,4根头刚毛较短(3~4 μm),化感器呈螺旋状(2.5~3.0 圈),交接刺细长,近端略膨大,中间开始至远端变细(65~69 μm),引带具直的引带突(20~25 μm),具 13~15 个小的乳突状肛前辅器;厦门后菱光线虫(Metachromadora xiamenensis Zhou,Zeng,Cai,Fu&Tan,2020)特征为:表皮具明显环纹,4 根头刚毛短且粗,脊线由食道中部延伸至尾中部,双环状化感器位于身体最前端,交接刺近端具头状膨大(58~65 μm),肛前辅器细管状(15 个),尾为短圆锥形.同时,获得了两种线虫核糖体大亚基(LSU)的D2-D3 片段序列数据,构建了两种线虫基于贝叶斯推论法(Bayesian inference,BI)和最大似然法(maximum likelihood,ML)的系统发育树,结果表明萨巴线虫属和后菱光线虫属表现出了多系进化的趋势.

Two dominant species,Sabatieria foetida Gagarin&Nguyen Vu Thanh,2008 and Metachromadora xiamenensis Zhou,Zeng,Cai,Fu,&Tan,2020,were redescribed during the classification and diversity studies of free-living marine nematodes in Dongtou Island,Zhejiang Province.S.foetida was first described as a recorded species and characterized by a cuticle with small punctuations and lateral differentiation with large dots,four short cephalic setae(3-4 μm in length),spiral-shaped amphidial fovea(2.5-3.0 turns),curved spicules with proximal ends that are cephalate and slender from the middle part,gubernaculum with straight apophysis(20-25 μm in length),and 13-15 papilliform precloacal supplements.Furthermore,M.xiamenensis is characterized by a coarsely striated cuticle with lateral alae starting from the middle pharynx to the tail,four short and stout cephalic setae,amphids with a double contour at the anterior end,spicules with a cephalated proximal end(58-65 μm in length),15 tubular precloacal supplements,and a short and conical tail.The D2-D3 fragments of the LSU rDNA of S.foetida and M.xiamenensis were amplified and sequenced.Moreover,phylogenetic trees based on Bayesian infer-ence and maximum likelihood revealed polyphyletic evolution in the genera Sabatieria and Metachromadora.

郭文;梁慧鑫;吕艳伟;王春明

聊城大学生命科学学院,山东 聊城 252059

生物学

丑萨巴线虫(Sabatieria foetida)厦门后菱光线虫(Metachromadora xiamenensis)LSU系统发育

Sabatieria foetidaMetachromadora xiamenensisLSUphylogeny

《海洋科学》 2024 (004)

45-52 / 8

国家自然科学基金项目(31100456)National Natural Science Foundation of China,No.31100456

10.11759/hykx20230311001

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