昆明医科大学学报2024，Vol.45Issue(6)：7-14,8.DOI:10.12259/j.issn.2095-610X.S20240602
1株悬浮培养的Vero细胞驯化及其生物学特性研究
Domestication and Biological Characteristics of a Vero Cell Line in Suspension Culture
摘要
Abstract
Objective To domesticate adherently cultured Vero cells into suspension-cultured cells and investigate their biological characteristics.Methods Cell culture was optimized using serum-free media and the"shaker adaptation method."Additionally,tumorigenicity,short tandem repeat(STR)profiles,morphological characters under transmission electron microscope(TEM),and influenza virus sensitivity were analyzed in these cells.Results Suspension cell line Vero-S was successfully established.The growth period of Vero-S cells ranged from 24 to 72 hours,with a plateau phase between 108 and 144 hours,and a decline phase after 144 hours.The population doubling time(PDT)was 36 hours,and cell density ranged from 14.01 to 14.88×108 cells/L.Average cell roundness ranged from 0.73 to 0.75,and cell diameter ranged from 12.12 to 14.44 µm.Vero-S cells formed tumors subcutaneously in nude mice,while Vero cells before suspension culture did not exhibit tumorigenicity.STR analysis indicated a primate origin of the cell line.Under electron microscopy,cell structure appeared intact.Vero-S cells showed insensitivity to influenza virus infection.Conclusion The established suspension cell line Vero-S exhibiting growth patterns similar to adherent cells,with uniform cell size and high-density growth supported by the established suspension culture medium.STR results demonstrated complete genetic stability of suspended Vero cells,with no apparent differences observed under transmission electron microscopy compared to adherent Vero cells.This study provides reference for suspension culture domestication techniques and can be applied in mechanistic studies of cell tumorigenicity.关键词
Vero细胞/细胞悬浮驯化技术/成瘤性/病毒敏感性Key words
Vero cells/Cell suspension domestication technology/Tumorigenicity/Virus sensitivity分类
农业科技引用本文复制引用
付兰博,阮朝列,陈昶旭,高羽,鲍晓琳,刘伯川,周健,李卫东..1株悬浮培养的Vero细胞驯化及其生物学特性研究[J].昆明医科大学学报,2024,45(6):7-14,8.基金项目
云南省重大科技专项基金资助项目(202302AA310002) （202302AA310002）
云南省基础研究专项基金资助项目(202301AU070048) （202301AU070048）
中国医学科学院医学与健康科技创新工程基金资助项目(2021-I2M-1-043) （2021-I2M-1-043）
协和实验室建设专项-基于大人群队列探索呼吸共病共性机制和干预手段研究基金资助项目(2023-I2M-2-001) （2023-I2M-2-001）
