山东农业科学2024，Vol.56Issue(6)：1-6,6.DOI:10.14083/j.issn.1001-4942.2024.06.001
番茄LeGlyⅠ基因的生物信息学特征与表达模式分析
Analysis on Bioinformatics Characteristics and Expression Pattern of LeGlyⅠ Gene in Tomato
摘要
Abstract
The plant may produce excessive toxic aldehydes such as methylglyoxal(MG)when facing adversity stress,and glyoxal enzyme system can effectively clear the excessive MG.Glyoxalase I(GlyⅠ)as the first enzyme in the cascade reaction plays an important role.In this study,LeGlyⅠgene was successfully cloned from the seedlings of tomato'Heinz 1706',and its bioinformatics characteristics,expression pattern and subcellular localization were analyzed.The results showed that the total length of LeGlyⅠCDS sequence was 489 bp,which encoding 162 amino acids.The LeGlyⅠ was predicted to be a hydrophilic stable protein with molecular formula as C798 H1226 N214 O235 S4,molecular weight as 17 706.05 Da,isoelectric point as 6.51,GRAVY coefficient as-0.386 and instability index as 39.32,and it had a VOC-like domain.The promoter se-quence of LeGlyⅠcontained a variety of cis-acting elements,including cis-acting elements necessary for anae-robic induction,light response elements,low-temperature response elements,drought response elements,and hormone response elements such as methyl jasmonate,abscisic acid,auxin and gibberellin.Through qRT-PCR analysis,it was found that LeGlyⅠexpressed in roots,stems and leaves of tomato with the highest expression level in leaves,and the expression could be affected by oxidative stress.The subcellular localization results showed that LeGlyⅠprotein was mainly localized in mitochondria and nucleus.These results could provide the-oretical bases for further research on the function and molecular mechanism of LeGlyⅠgene in tomato resistant to stresses.关键词
LeGlyⅠ基因/番茄/生物信息学分析/氧化胁迫/基因表达/亚细胞定位Key words
LeGlyⅠ gene/Tomato/Bioinformatics analysis/Oxidative stress/Gene expression/Subcel-lular localization分类
农业科技引用本文复制引用
孙晓辉,张月昌,蒋健,石勇志,孙秀东..番茄LeGlyⅠ基因的生物信息学特征与表达模式分析[J].山东农业科学,2024,56(6):1-6,6.基金项目
国家自然科学基金面上项目(31772323) （31772323）
