松树赤枯病玫瑰拟盘多毛孢菌的鉴定及生物学特性研究OA北大核心CSTPCD
Identification and Biological Characteristics of Pestalotiopsis rosea Causing Pine Needle Blight
本研究对从山东威海双岛林场采集的松树枯萎样本进行分离培养、单孢纯化、致病性测定,得到一株能引起松树赤枯病的病原菌,编号为RCBHL.经形态学观察和多基因(ITS、TEF-1、TUB2)系统发育分析,将菌株鉴定为玫瑰拟盘多毛孢菌(Pestalotiopsis rosea).生物学特性测定结果表明,该菌株最适生长温度为20～25℃,最优生长培养基为PDA培养基,最适生长pH值为 6.0,最适光照条件为 24h光照.该研究结果可为松树赤枯病的防治提供理论依据.
In this experiment,the samples of pine needle blight were collected from the Shuangdao For-est Farm in Weihai,Shandong Province,and a pathogenetic strain RCBHL was obtained after separation and culture,single spore purification and pathogenicity identification test.According to morphological characteris-tics and multilocus phylogenetic(ITS,TEF-1 and TUB2)analyses,it was identified as Pestalotiopsis rosea.The testing results of biological characteristics showed that the optimum cultural conditions for P.rosea RCHBL included 20～25℃,6.0 of pH value,PDA culture medium and 24 h light.The research results would provide the theoretical basis for prevention and control of pine needle blight.
韩凤英;亓玉昆;杨慧;秦永梅;周丽颜;李星仪;李海燕;刘敏;王清海
山东农业工程学院,山东 济南 250100山东省林业科学研究院,山东 济南 250014济南市公园发展服务中心,山东 济南 250001
林学
松树赤枯病分离纯化病原鉴定生物学特性
Pine needle blightSeparation and purificationPathogen identificationBiological charac-teristics
《山东农业科学》 2024 (006)
113-119 / 7
中央林业改革发展资金防灾减灾项目(鲁财资环指[2021]27号);2022年山东省本科教学改革研究项目(Z2022211);山东省"黄河流域生态保护和教育高质量发展"教育教学专项课题(2022HHZX005)
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