细菌性阴道病患者阴道微环境中差异蛋白的蛋白组学研究OACSTPCD

Objective:To explore key proteins in the vaginal microenvironment of bac-terial vaginosis(BV)using proteomics and mass spectrometry techniques.Method:The vaginal secretions of 10 BV women and 10 healthy women who visited the outpatient and physical exam-ination centers of Peking University First Hospital from January 2019 to October 2019 were col-lected.Differential protein analysis techniques were used to screen for differential proteins,and key proteins related to BV were screened for GO(Gene Ontology)enrichment,KEGG(Gene Ontology Enrichment Analysis)enrichment,and protein protein interaction(PPI)enrichment.Result:2136 humanized proteins were identified,including 142 differentially expressed pro-teins,104 upregulated proteins,and 38 downregulated proteins.The biological processes in-volved mainly include protein folding,protein demethylation,cytoplasmic translation,and protein binding.760 bacterial derived proteins were identified,including 56 differential proteins,32 up-regulated and 24 downregulated.The biological processes involved mainly include glucose me-tabolism,glycolysis,membrane transport,and ATP hydrolysis.The content of enolase in vaginal secretions of BV patients was significantly higher than that of healthy women,and the difference was statistically significant(P<0.05).Conclusion:Proteomics and mass spectrometry tech-niques can screen for relevant proteins and signaling pathways that may be involved in the path-ogenesis of BV,such as enolase,providing important information for elucidating its occurrence and development process,and potentially providing important targets for clinical diagnosis and treatment.