现代妇产科进展2024，Vol.33Issue(7)：488-492,500,6.DOI:10.13283/j.cnki.xdfckjz.2024.07.002
细菌性阴道病患者阴道微环境中差异蛋白的蛋白组学研究
Proteomic study on differential proteins in the vaginal microenvironment of patients with bacterial vaginosis
摘要
Abstract
Objective:To explore key proteins in the vaginal microenvironment of bac-terial vaginosis(BV)using proteomics and mass spectrometry techniques.Method:The vaginal secretions of 10 BV women and 10 healthy women who visited the outpatient and physical exam-ination centers of Peking University First Hospital from January 2019 to October 2019 were col-lected.Differential protein analysis techniques were used to screen for differential proteins,and key proteins related to BV were screened for GO(Gene Ontology)enrichment,KEGG(Gene Ontology Enrichment Analysis)enrichment,and protein protein interaction(PPI)enrichment.Result:2136 humanized proteins were identified,including 142 differentially expressed pro-teins,104 upregulated proteins,and 38 downregulated proteins.The biological processes in-volved mainly include protein folding,protein demethylation,cytoplasmic translation,and protein binding.760 bacterial derived proteins were identified,including 56 differential proteins,32 up-regulated and 24 downregulated.The biological processes involved mainly include glucose me-tabolism,glycolysis,membrane transport,and ATP hydrolysis.The content of enolase in vaginal secretions of BV patients was significantly higher than that of healthy women,and the difference was statistically significant(P<0.05).Conclusion:Proteomics and mass spectrometry tech-niques can screen for relevant proteins and signaling pathways that may be involved in the path-ogenesis of BV,such as enolase,providing important information for elucidating its occurrence and development process,and potentially providing important targets for clinical diagnosis and treatment.关键词
烯醇化酶/细菌性阴道病/阴道加德纳菌/阴道乳杆菌Key words
Enolase/Bacterial vaginosis/Gardnerella vaginalis/Lactobacillus vaginalis分类
医药卫生引用本文复制引用
张瑞,李昶,马爱昕,刘朝晖..细菌性阴道病患者阴道微环境中差异蛋白的蛋白组学研究[J].现代妇产科进展,2024,33(7):488-492,500,6.基金项目
国家自然科学基金资助项目(No:81471420) （No:81471420）
教育部博士点新教师基金项目(No:20130001120051) （No:20130001120051）
