中国兽医科学2024，Vol.54Issue(7)：859-865,7.DOI:10.16656/j.issn.1673-4696.2024.0110
CpG和poly I∶C增强口蹄疫病毒样颗粒疫苗免疫效果的研究
Enhancing immune effect of foot-and-mouth disease virus-like particles vaccine with CpG and poly I∶C
摘要
Abstract
This study was to evaluate the immune-enhancing effects of CpG and poly I∶C on the foot-and-mouth disease(FMD)virus-like particles(VLPs)vaccine.Using purified serotype 0 FMD VLPs as anti-gens,the mice bone marrow-derived dendritic cells(BMDC)were stimulated with VLPs-CpG and VLPs-poly I:C followed by the expression levels of CD40 and CD86 in BMDC was analyzed using flow cytometry.Fur-ther,VLPs,VLPs-CpG and VLPs-poly I∶C were emulsified with ISA206 to immunize guinea pigs.The improve-ment of CpG and poly I∶C on immune effect of the VLPs vaccine was evaluated by detecting the specific an-tibodies,neutralizing antibodies and splenic lymphocyte proliferation,as well as virus challenge.Re-sults showed that the expression levels of CD86 in VLPs-CpG and VLPs-poly I∶C were higher than VLPs.The adjuvant ISA206 combined with poly I∶C and CpG could improve the protection against virus.ISA206 com-bined with CpG accelerated the responses of specific antibodies and neutralizing antibodies,as well as enhanced the proliferation of splenic lymphocytes.This study demonstrates both CpG and poly I∶C has application potential to enhance the immune effect of FMD VLPs vaccine.关键词
口蹄疫/病毒样颗粒/CpG/poly I∶C/免疫效果Key words
foot-and-mouth disease(FMD)/virus-like particles(VLPs)/CpG/poly I∶C/immune efficacy分类
农业科技引用本文复制引用
任丽华,罗新,董虎,谭书桢,郭慧琛,孙世琪,魏彦明,滕志东..CpG和poly I∶C增强口蹄疫病毒样颗粒疫苗免疫效果的研究[J].中国兽医科学,2024,54(7):859-865,7.基金项目
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
国家重点研发计划项目(2021YFD1800300,2022YFD1800603) （2021YFD1800300,2022YFD1800603）
国家自然科学基金项目(32072847,32072859,32301127 （）
兰州市人才创业创新项目(2023-RC-3) （2023-RC-3）
甘肃省自然科学基金项目(22JR5RA032,23JRRA551) （22JR5RA032,23JRRA551）
