中国兽医科学2024，Vol.54Issue(7)：915-920,6.DOI:10.16656/j.issn.1673-4696.2024.0094
布鲁氏菌量子点荧光微球免疫层析试纸条的研制
Development of a quantum dot fluorescent microspheres immunochromatographic test strip for Brucella detection
摘要
Abstract
To prepare a Brucella immunochromatographic strip based on quantum dot fluorescent microspheres,Brucella lipopolysaccharide monoclonal antibodies and goat anti-mouse IgG antibodies were sprayed onto nitrocellulose membranes as detection and quality control lines,respectively.Brucella lipopolysaccharide monoclonal antibodies were labeled with quantum dot fluorescent microspheres,diluted,and sprayed onto glass cellulose membranes.Finally,assembly and cutting were performed to produce test strips for detection,and their sensitivity,specificity,and stability were measured.Finally,using Brucella fluorescence quantitative PCR detection technology as a control,120 clinical samples were tested using this test strip to compare the compliance rates of the two methods.Results,the established quantum dot fluorescent microsphere immunochromatographic test strip showed good performance,with a detection sensitivity of 1 ng/mL for brucellosis and good specificity.There was no cross reaction with other pathogens.The stability test found that the product had good stability when stored at room temperature for 18 months or 37 ℃ for 28 d,with coefficients of variation of 3.2％and 14.6％,respectively;120 clinical samples were tested using test strips and fluorescence quantitative PCR methods.Compared with the results of fluorescence quantitative PCR,the positive conformity rate was 91％,the negative conformity rate was 100％,and the total conformity rate was 99％.Conclusion,the test strip has the advantages of simple operation,rap id stability,high sensitivity and low cost.The detection can be completed within 20 minutes,and the sample acquisition is easy.It can achieve rapid on-site detection and has good prospects in the pathogen detection and purification of brucellosis.关键词
布鲁氏菌/量子点荧光微球/免疫层析技术Key words
Brucella/quantum dot fluorescent microspheres/immunochromatography technolog分类
农业科技引用本文复制引用
许玉静,杨若松,李翀,华利忠,杨卫军..布鲁氏菌量子点荧光微球免疫层析试纸条的研制[J].中国兽医科学,2024,54(7):915-920,6.基金项目
河北省重点研发计划项目(21327601D) （21327601D）
