多维度评价及优化黄芪瞬时高温灭菌工艺OA北大核心CSTPCD

In order to optimize the high temperature short time(HTST)process parameters of Astragalus membranaceus and investigate the effects on the quality of Astragalus membranaceus from many angles,based on the criteria importance though intercrieria correlation(CRITIC)method,orthogonal design was used to optimize the sterilization process parameters of Astragalus membranaceus.The sterilization temperature,sterilization time and fineness of pulverization of the medicinal materials were inspected,and the sterilization rate,the content of 5 compounds and the free radical clearance rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH·)were determinated.The effects of sterilization on the 3 indexes were evaluated by intuitive and variance analysis.The structure of common peaks was confirmed by liquid chromatography-mass spectrometry(LC-MS).Partial least square regression analysis was used to analyze the relationship between flavonoids and antioxidant activity of common peaks.The results showed that the sterilization temperature was a significant factor(P＜0.05).The optimal sterilization process parameters were determined as:sterilization temperature(170±2)℃,sterilization time 5 s,fineness of pulverization 50 mesh.The microbial standard test of the 3 batches of samples after sterilization according to the optimized process all met the requirements of Pharmacopoeia of the People's Republic of China.The contents of 5 compounds and DPPH·clearance rate had no significant change compared with that before sterilization.The similarity of fingerprints before and after sterilization was greater than 0.900,and the correlation results of spectral dynamic analysis were basically consistent with the experimental results and structural characteristics of the compounds.In conclusion,high temperature short time had obvious killing effect on microorganism in Astragalus membranaceus,but had no obvious effect on their quality,indicating that this method was suitable for the sterilization of Astragalus membranaceus.