中国医科大学学报2024，Vol.53Issue(7)：591-596,6.DOI:10.12007/j.issn.0258-4646.2024.07.003
MFG-E8抑制青光眼大鼠视网膜神经节细胞凋亡的机制研究
Mechanism of MFG-E8 inhibiting apoptosis of retinal ganglion cells in glaucoma rats
摘要
Abstract
Objective To investigate the role and mechanism of milk fat globule-EGF factor 8(MFG-E8)in neuroprotection in glaucoma rats.Methods A glaucoma rat model was constructed,and MFG-E8 or D89E was injected into the vitreous cavity of the rats.The intraoc-ular pressure of the rats was measured.HE staining,TUNEL staining and immunofluorescence staining were used to detect the patholog-ical injury,apoptosis and microglia activation of the retina.The protein expressions of cleaved caspase-3,caspase-3,cleaved caspase-9,caspase-9 and Bax were detected by Western blotting,and the expression levels of IL-10,TGF-βand NGFwere detected by ELISA and RT-qPCR.Results Compared with the control group,the intraocular pressure of glaucoma rats significantly increased,the retinal GCC layer became thinner,the apoptotic cells increased,the levels of cleaved caspase-3/caspase-3,cleaved caspase-9/caspase-9 and Bax increased,and the number of IBA1 positive cells increased,after treatment with MFG-E8,retinal GCC layer thickness increased,and the levels of cleaved caspase-3/caspase-3,cleaved caspase-9/caspase-9,Bax decreased,the number of IBA1 positive cells and the levels of IL-10,TGF-β,NGFincreased,but the progression of glaucoma was aggravated after the treatment of MFG-E8 inactivated isomer D89E.Conclusion MFG-E8 can mediate microglia activation,inhibit apoptosis of ganglion cells,and slow down the progression of glaucoma in rats.关键词
青光眼/乳脂肪球表皮生长因子8/视网膜神经节细胞/小胶质细胞Key words
glaucoma/milk fat globule-EGF factor 8/retinal ganglion cell/microglia分类
医药卫生引用本文复制引用
杨静,曾明兵,杨军,史贻玉,陈海波..MFG-E8抑制青光眼大鼠视网膜神经节细胞凋亡的机制研究[J].中国医科大学学报,2024,53(7):591-596,6.基金项目
海南省自然科学基金青年基金(820QN414) （820QN414）
海南省卫生健康行业科研项目(20A200322) （20A200322）
