中国食品添加剂2024，Vol.35Issue(6)：86-92,7.DOI:10.19804/j.issn1006-2513.2024.6.011
小球藻生长因子对UVA诱导人皮肤成纤维细胞紫外损伤的保护作用研究
Protective effect of Chlorella growth factor on UVA induced ultraviolet damage of human skin fibroblasts
摘要
Abstract
The nutrients composition of Chlorella growth factor(CGF)was determined by phenol-sulfuric acid method,Coomassie brilliant blue method and ultraviolet spectrophotometry.The protective effect of CGF on ultraviolet-injured human skin fibroblasts(HSF)was determined by MTT method and the expression of collagen-related factors were determined by enzyme-linked immunosorbent(ELISA),respectively.The results showed that nucleic acids accounted for 84.40%±0.73%of CGF.Pretreatment with Chlorella Growth Factor significantly reduced the proliferation inhibition of HSF cell by UVA.Compared with the model group,treatment with a 50 μg/mL concentration of CGF increased HSF cell viability by 75.05%,showing a dose-dependent relationship within 5～50μg/mL of CGF.ELISA results revealed that Chlorella growth factor could increase the expression of tissue inhibitors of metalloproteinase-1(TIMP-1)and type I collagen(COL-I)in HSF cells and reduce the expression of matrix metalloproteinase-1(MMP-1).Chlorella growth factor is an excellent exogenous nucleotide nutrient that can effectively alleviate the damage caused by UVA exposure,possibly through promoting collagen synthesis.关键词
小球藻生长因子/核苷酸含量/人皮肤成纤维细胞/紫外损伤模型/I型胶原蛋白Key words
Chlorella growth factor/nucleotides content/human skin fibroblast/UV damage model/type I collagen分类
轻工纺织引用本文复制引用
何亚玲,苏桂惠,唐振洲,苏志维..小球藻生长因子对UVA诱导人皮肤成纤维细胞紫外损伤的保护作用研究[J].中国食品添加剂,2024,35(6):86-92,7.基金项目
广西科技计划项目重点研发项目(桂科AB19259010) （桂科AB19259010）
广西中医药大学"桂派杏林青年英才"项目(2022C039) （2022C039）
国家自然科学基金项目(32060098) （32060098）
广西中医药大学海洋药物研究院团队科研专项(2018ZD005-A08) （2018ZD005-A08）
