中国合理用药探索2024，Vol.21Issue(6)：33-39,7.DOI:10.3969/j.issn.2096-3327.2024.06.006
两株携带blaNDM基因肠杆菌目细菌的分子流行病学特征研究
Molecular Epidemiological Characterization of Two Strains of blaNDM-positive Enterobacteriaceae
摘要
Abstract
Objective:To understand the molecular epidemiological characteristics of two clinical isolates of blaNDM-positive Enterobacteriaceae in a hospital in Pingdingshan city so as to provide theoretical support for the prevention and control of nosocomial infection caused by this kind of pathogens.Methods:Clinical isolates of carbapenem-resistant Enterobacteriaceae(CRE)from January to December in 2022 were collected.The blaNDM-positive Enterobacteriaceae were screened using polymerase chain reaction(PCR),and other drug resistance genes[including genes resistant to carbapenems,extended-spectrum β-lactamases(ESBLs)and quinolones]carried by the strains were subsequently amplified and sequenced,followed by multilocus sequence typing(MLST)to analyze phylogenetic relationship among the strains of the same species.Results:Among the collected CRE,2 strains carried blaNDM genes and were identified as Citrobacter freundii with blaNDM-1 and Escherichia coli with blaNDM-5.These two strains both carried multiple drug-resistant genes and were resistant to multiple common clinical medicines.The MLST result was ST85 for NDM-1-producing Citrobacter freundii and ST736 for NDM-5-producing Escherichia coli,and neither wasa common epidemic clonal type.Southern blot analysis with blaNDM probe showed that blaNDM-1 gene was located on a plasmid with size of～54 kb,and blaNDM-5 gene was located on a plasmid with size of～104 kb.These two blaNDM-carrying plasmids could not be conjugated.Conclusion:CRE strains carrying blaNDM genes were reported in Pingdingshan city for the first time.The two reported strains both harbored multiple drug-resistant genes.And blaNDM genes were located on plasmids and related to a mobile element of insertion sequence(IS).It is suggested that surveillance,prevention and control of nosocomial infection should be strengthened to prevent the spread and prevalence of drug-resistant strains.关键词
耐碳青霉烯类肠杆菌目细菌/新德里金属β-内酰胺酶(NDM)/感染/流行病学/耐药基因Key words
carbapenem-resistant Enterobacteriaceae/New Delhi metallo-β-lactamase(NDM)/infection/epidemiology/drug-resistant genes分类
医药卫生引用本文复制引用
王健,李轶,白振宇,崔世平..两株携带blaNDM基因肠杆菌目细菌的分子流行病学特征研究[J].中国合理用药探索,2024,21(6):33-39,7.基金项目
2023年度河南省医学科技攻关计划联合共建项目(LHGJ20230021) （LHGJ20230021）
