江西农业大学学报2024，Vol.46Issue(3)：638-647,10.DOI:10.3724/aauj.2024056
芫花叶片和茎段离体再生体系的构建
Construction of an in vitro regeneration system for the leaves and stem segments of Daphne genkwa
摘要
Abstract
[Objective]Daphne genkwa is a well-known native flower and traditional medicinal plant with significant ecological,ornamental,and medicinal value.Researching and addressing the rapid propagation methods for D.genkwa is an essential requirement for its conservation and utilization.[Method]Using leaves and stem segments of robust D.genkwa plants as explants,we investigated the effects of different sterilization methods on D.genkwa explants.We also studied the effects of different hormone ratios on the induction of callus tissues,the proliferation of adventitious shoots,and the rooting of D.genkwa Our aim was to construct a highly efficient regeneration system for D.genkwa explant tissues.[Result]The explants of D.genkwa were disinfected with 75%ethanol solution for 25-35 second,2%NaClO solution and 0.1%HgCl2 solution for 15 min,The contamination rate of D.genkwa stem segment explants was 52.7%,while the leaf explants was 49.0%.MS+0.8 mg/L 6-BA+0.35 mg/L NAA was the best primary induction medium for D.genkwa stem segment explants,with an 79.7%tissue induction rate and 96.4%induced adventitious shoots.MS+0.8 mg/L IBA+0.85 mg/L NAA was the best primary induction medium for D.genkwa leaf explants,with a 99.8%tissue induction rate and 94.5%induced adventitious shoots.MS+0.35 mg/L 6-BA+0.35 mg/L IBA was the best successional proliferation medium for D.genkwa stem segments and leaves,with the highest proliferation coefficient of 7.36 and the highest average shoot height of 2.47 cm.MS+0.6 mg/L IBA+1.0 mg/L NAA+0.08 g/L ascorbic acid was the best rooting medium for D.genkwa stem explants,with an average rooting of 3.67 and root length of 2.16 cm;MS+0.6 mg/L IBA+0.6 mg/L NAA+0.07 g/L ascorbic acid was the best rooting medium for leaf explants of D.genkwa,with an average of 1.33 roots and a root length of 1.95 cm.[Conclusion]An isolated tissue regeneration system was developed using D.genkwa leaves and stem segments as explants.This system showed high rates of tissue healing induction,stimulated adventitious shoots,proliferation coefficients,and rooting rates.It effectively addressed the technical challenges related to efficient D.genkwa seedling propagation.关键词
组织培养/叶片/茎段/再生体系/芫花Key words
Tissue culture/leaf/stem segment/regeneration system/Daphne genkwa分类
农业科技引用本文复制引用
冯晓涛,戴小英,李康琴,于丽欣,高丽琴,邓绍勇..芫花叶片和茎段离体再生体系的构建[J].江西农业大学学报,2024,46(3):638-647,10.基金项目
江西省重点研发计划项目(20203BBGL73219)Project supported by the Key R&D Program Project of Jiangxi Province(20203BBGL73219)江西省"科技+水利"联合计划项目(2022KSG01001)、中药材芫花栽培技术规程(2023A07)同时对本研究给予了资助,谨致谢意! （20203BBGL73219）
