药学研究2024，Vol.43Issue(6)：544-548,572,6.DOI:10.13506/j.cnki.jpr.2024.06.004
桑藿排毒茶体外抗甲型H1N1流感病毒机制分析
Analysis of the anti influenza A(H1N1)virus mechanism of Sanghuo Paidu Tea in vitro
摘要
Abstract
Objective To observe the inhibitory effect of Sanghuo Paidu Tea on influenza A(H1N1)virus at the cellular level.Methods CCK-8 reagent was used to detect the cytotoxicity of Sanghuo Paidu Tea,and the anti-influenza A virus activity of Sanghuo Paidu Tea was evaluated by analyzing the changes in the expression levels of influenza A virus RNA and protein in different treatment groups.Results The maximal atoxic concentration(TC0)of Sanghuo Paidu Tea and Oseltamivir phosphate for MDCK was 4.32 mg·mL-1 and 1.02 mg·mL-1,respectively,and the 50%effective concentration(EC50)was 0.38 mg·mL-1 and 3.5 μg·mL-1.In Q-PCR and Western blotting experiments,compared to the viral group,the expression levels of influenza virus RNA and protein in infected cells treated with drugs were significantly reduced,indicating a strong inhibitory effect on the virus.Conclusion Sanghuo Paidu Tea can resist the infection of influenza A virus at the cellular level,and has further development significance.关键词
桑藿排毒茶/甲型流感病毒/抗病毒作用/奥司他韦Key words
Sanghuo Paidu Tea/Influenza A virus/Antiviral effect/Oseltamivir分类
中医学引用本文复制引用
邵秀丽,何峰..桑藿排毒茶体外抗甲型H1N1流感病毒机制分析[J].药学研究,2024,43(6):544-548,572,6.基金项目
湖北省重点研发计划项目(No.2022BCE013) （No.2022BCE013）
