桑藿排毒茶体外抗甲型H1N1流感病毒机制分析OACSTPCD

Objective To observe the inhibitory effect of Sanghuo Paidu Tea on influenza A(H1N1)virus at the cellular level.Methods CCK-8 reagent was used to detect the cytotoxicity of Sanghuo Paidu Tea,and the anti-influenza A virus activity of Sanghuo Paidu Tea was evaluated by analyzing the changes in the expression levels of influenza A virus RNA and protein in different treatment groups.Results The maximal atoxic concentration(TC0)of Sanghuo Paidu Tea and Oseltamivir phosphate for MDCK was 4.32 mg·mL-1 and 1.02 mg·mL-1,respectively,and the 50%effective concentration(EC50)was 0.38 mg·mL-1 and 3.5 μg·mL-1.In Q-PCR and Western blotting experiments,compared to the viral group,the expression levels of influenza virus RNA and protein in infected cells treated with drugs were significantly reduced,indicating a strong inhibitory effect on the virus.Conclusion Sanghuo Paidu Tea can resist the infection of influenza A virus at the cellular level,and has further development significance.