山东中医杂志2024，Vol.43Issue(7)：694-699,6.DOI:10.16295/j.cnki.0257-358x.2024.07.007
基于16sRNA测序技术探讨不同体质腹型肥胖者肠道菌群特征
Exploring Characteristics of Gut Microbiota in Subjects with Abdominal Obesity of Different Constitutions Based on 16sRNA Sequencing Technology
摘要
Abstract
Objective:To analyze the diversity and species variations of gut microbiota in people with phlegm and dampness constitution and with non phlegm and dampness constitution.Methods:Ninety-one fecal samples were gathered.Among them,44 subjects were with phlegm dampness constitution and 47 were with non phlegm dampness constitution.The Illumina MiSeq 6000 high-throughput sequencing platform was applied to examine the gut microbiota's relative abundance,species diversity in individuals with phlegm dampness constitution and with non phlegm dampness constitution.Results:There were obvious differences in gut microbiota in terms of relative prevalence of microbiota across five phylum levels between the phlegm dampness constitution and the non phlegm dampness constitution,with statistical signifinace(P＜0.05),and the differences between in Bacteroides,Spirillum and Enterobacteriaceae were more obvious(P＜0.05).Conclusion:There are differences in gut microbiota between abdominal obesity subjects with phlegm dampness constitution and those with non phlegm dampness constitution,and the difference in microbiota may be an inherent factor in the formation of constitution differences.关键词
中医体质/痰湿体质/非痰湿体质/肠道菌群/16sRNA/腹型肥胖/Alpha多样性Key words
traditional Chinese medicine constitution/phlegm and dampness constitution/non phlegm and dampness constitution/gut microbiota/16sRNA/abdominal obesity/Alpha diversity分类
中医学引用本文复制引用
袁梦华,扈新刚,赵燕..基于16sRNA测序技术探讨不同体质腹型肥胖者肠道菌群特征[J].山东中医杂志,2024,43(7):694-699,6.基金项目
国家重点研发计划项目(编号:2019YFC1710102) （编号:2019YFC1710102）
