生命科学研究2024，Vol.28Issue(3)：223-231,9.DOI:10.16605/j.cnki.1007-7847.2023.03.0141
CRISPR/Cas9系统递送方式的发展及其在昆虫研究中的应用
Development of CRISPR/Cas9 System Delivery Methods and Their Applications in Insect Research
摘要
Abstract
Since the emergence of the CRISPR/Cas9 gene editing system,there has been significant ad-vancement in understanding biological gene functions.CRISPR/Cas9 is typically delivered via microinjection into insect embryos for gene editing.However,due to diverse reproductive modes in insects,it is difficult to use traditional CRISPR/Cas9 delivery methods to study the gene functions of some insect species,such as cockroaches with rigid egg cases and viviparous aphids.In the current CRISPR/Cas9 technology,alternative delivery methods have been developed,which do not require injection into early-stage insect embryos and have been reported as ReMOT(receptor-mediated ovary transduction)and DIPA-CRISPR("direct parental"CRISPR).These methods allow the direct injection of editing reagents into the hemocoel of adult female in-dividuals,introducing genetic mutations effectively into developing oocytes and simplifying the gene editing process.They have been successfully applied to the study of insects such as Aedes aegypti,Blattella ger-manica,and Tribolium castaneum.This article reviews the structure,mechanism,and traditional delivery methods of the CRISPR/Cas9 system,as well as the application and prospects of the newly developed Re-MOT and DIPA-CRISPR delivery methods in insect research,aiming to provide guidance for gene editing in more species.关键词
CRISPR/Cas9/ReMOT/DIPA-CRISPR/基因编辑/昆虫Key words
CRISPR/Cas9/ReMOT/DIPA-CRISPR/gene editing/insect分类
生物学引用本文复制引用
王露,阮永明..CRISPR/Cas9系统递送方式的发展及其在昆虫研究中的应用[J].生命科学研究,2024,28(3):223-231,9.基金项目
浙江省自然科学基金项目(LGN18C040001) （LGN18C040001）
