食品工业科技2024，Vol.45Issue(14)：15-24,10.DOI:10.13386/j.issn1002-0306.2023100175
枸杞叶蛋白提取物的特性表征与生物活性评价
Characterisation and Bioactivity Evaluation of Lycium barbarum Leaf Protein Extract
摘要
Abstract
In order to investigate the characterisation and biological activity of the leaf protein extract of Lycium barbarum,alkaline extraction and acid precipitation method was used to extract Lycium barbarum leaf protein extract.And the physicochemical properties,functional properties,structural characterisation and bioactivities of Lycium barbarum leaf protein extract were analysed by the methods of water holding capacity(WA),oil-holding property(FA),thermal stability(TM),solubility(PS),froth capability(FC),emulsification capability(EC),and other indexes as well as by the sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE),scanning electron microscopy(SEM),ultraviolet-visible spectroscopy(UV),Fourier infraredspectroscopy(FT-IR),and X-ray Diffraction(XRD),and the in vitro radical scavenging assays and enzyme inhibition assays.The results showed that the total amino acid content of Lycium barbarum leaf protein extract reached 344.00±10.49 mg/100 g,the molecular weight ranged in 40 to 55 kDa,the water WA and FA property were 2.70 and 3.43 g/g,and the denaturation temperature was 85.73 ℃.With the increase of pH,the PS,FC,EC and froth stability(FS)of Lycium barbarum leaf protein extract showed a tendency of first decreasing and then increasing,while emulsion stability(ES)was exactly the opposite.The surface of Lycium barbarum leaf protein extract had tightly connected pores and was in the form of lumps of uneven sizes,with characteristicabsorption peaks near 270 nm,appearing amide A,B,Ⅰ,Ⅱ,Ⅲ bands,and a complete triplehelix structure.The scavenging rate of superoxide anion and hydroxyl radical was 37.73％and 35.38％at the concentration of 10 mg/mL of Lycium barbarum leaf protein extract,respectively.The IC50 of a-glucosidase and a-amylase was 9.88 and 21.09 mg/mL,respectively.In conclusion,the Lycium barbarum leaf protein extract was characterised and structurally stable,had certain in vitro antioxidant capacity and could inhibit the activity of enzymes related to blood glucose metabolism.This experiment provides theoretical basis for the development,utilisation and deep processing of Lycium barbarum leaf protein extract.关键词
枸杞叶/蛋白质/功能特性/抗氧化/酶活抑制Key words
Lycium barbarum leaf/protein/functional characteristics/antioxidant/enzyme inhibition分类
轻工纺织引用本文复制引用
杨雪,马麦迈,马利,余君伟,傅婧,范艳丽..枸杞叶蛋白提取物的特性表征与生物活性评价[J].食品工业科技,2024,45(14):15-24,10.基金项目
宁夏重点研发项目(2022BBF03016). （2022BBF03016）
