食品工业科技2024，Vol.45Issue(14)：121-130,10.DOI:10.13386/j.issn1002-0306.2023080217
猴头菌产酚类物质液态培养条件优化及对乳酸菌生长的影响
Optimization of Liquid Culture Conditions for Phenolic Substances Produced by Hericium erinaceus and Its Influence on the Growth of Lactic Acid Bacteria
马永强 1梁珊珊 1马宏良 1王瑞琦 1王植朔 1那治国 1徐伟1
作者信息
- 1. 哈尔滨商业大学食品工程学院,黑龙江省谷物食品与谷物资源综合加工重点实验室,黑龙江哈尔滨 150028
- 折叠
摘要
Abstract
Objetive:Culturing liquid hyphae with Hericium erinaceus pure strain.The medium was optimized to increase the content of Hericium erinaceus mycelium phenolic extracts.The composition of Hericium erinaceus mycelium phenolic extracts was analyzed,and the effects of phenolic extracts from Hericium erinaceus mycelium on lactic acid bacteria were also studied.Methods:Orthogonal design was used to study the effect on the total phenol content in liquid mycelium,and HPD-826 macroporous adsorption resin was used to purify it,and then HPLC-MS was used to determine its composition.Finally,adding the purified product to the lactic acid bacteria culture mediumm,and detected the effect on the number of viable lactic acid bacteria and its acid-production ability.Results:When the carbon source potato was 14％,nitrogen source peptone was 2％and inorganic salt MgSO4 was 0.3％,the total phenol content of Hericium erinaceus mycelium reached 0.783 mg/g.Sixteen monomeric phenols were identified from the phenolic extract of Hericium erinaceus mycelium,accounting for 88.27％.The main monomeric phenols were gallic acid-O-glucoside,p-coumaric acid-O-gallic acid glucoside,ferulic acid,catechin benzyl sulfide,gallic acid and caffeic acid.Adding phenol extraction solution from Hericium erinaceus mycelium to MRS medium of Lactobacilluscasei,Lactobacillus acidophilus and Streptococcus intestinalis could increase the number of viable bacteria of three kinds of lactic acid bacteria.Conclusion:Phenolic extract from Hericium erinaceus mycelium can promote the growth of lactic acid bacteria and provide new resource raw materials for functional food.关键词
液态培养猴头菌丝体/酚类提取物/液质联用/乳酸菌Key words
liquid culture of Hericium erinaceus mycelium/phenolic extract/HPLC-MS/lactic acid bacteria分类
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马永强,梁珊珊,马宏良,王瑞琦,王植朔,那治国,徐伟..猴头菌产酚类物质液态培养条件优化及对乳酸菌生长的影响[J].食品工业科技,2024,45(14):121-130,10.
