食品工业科技2024，Vol.45Issue(14)：131-138,8.DOI:10.13386/j.issn1002-0306.2023080245
L.plantarum SGJ-24全基因组测序及活性相关基因的挖掘和分析
Whole Genome Sequencing of L.Plantarum SGJ-24 and Mining and In-depth Analysis of Activity Related Genes
摘要
Abstract
To comprehensively understand the genetic information of Lactiplantibacillus plantarum SGJ-24 and thoroughly analyze the functional genes associated with its tolerance to challenging vinous environments,reduction of biological acid,and biogenic amine degradation activities.The study was conducted using whole-genome sequencing,which combined second-generation Illumina HiSeq technology with third-generation PacBio RS Ⅱ technology.The results showed that,a total genome size of 3451191 bp and 3361 coding genes.Numerous genes were annotated for functions such as nutrient(sugar,amino acid and so on)transport and metabolism,protein synthesis,and nucleic acid metabolism.Additionally,specific functional genes related to the versatile activities of this strain were identified,including malolactic fermentation activity(MLE and so on),acid tolerance(gadB and so on),ethanol tolerance(PFK,GK and so on),and biogenic amine degradation(GAPDH)function.The research findings provided a deep insight into the genetic characteristics of L.plantarum SGJ-24,laying a theoretical foundation for further development and utilization of the strain.关键词
植物乳杆菌/全基因组测序/功能基因/活性Key words
Lactiplantibacillus plantarum/whole genome sequencing/functional gene/activity分类
轻工纺织引用本文复制引用
褚琪,张艳芳,周浩然,邢鑫,孙舒扬..L.plantarum SGJ-24全基因组测序及活性相关基因的挖掘和分析[J].食品工业科技,2024,45(14):131-138,8.基金项目
国家自然科学基金面上项目(32272281) （32272281）
山东省高等学校青年创新团队发展计划(食品纳米技术创新团队) （食品纳米技术创新团队）
山东省自然科学基金面上项目(ZR2020MC204). （ZR2020MC204）
