食品工业科技2024，Vol.45Issue(14)：344-351,8.DOI:10.13386/j.issn1002-0306.2023080238
紫苏叶总黄酮对APAP诱导的急性肝损伤的保护作用
Protective Effect of Total Favonoids of Perilla frutescens on APAP-Induced Acute Liver Injury
摘要
Abstract
Objective:To study the best purification method of Perilla flavone(PF),and to investigate its protective effect and potential mechanism on Paracetamol(APAP)-induced acute liver injury.Methods:Single factor experiments were used to determine the optimal conditions for the purification of PF by macroporous resin.BALB/c male mice were randomly divided into 6 groups,blank control group(CON),model group(MOD),diphenylene dibenzoate group(DDB),low dose group of PF(L-PF,12.5 mg/kg),middle dose group of PF(M-PF,25 mg/kg),and high dose group of PF(H-PF,50 mg/kg).DDB and PF were given by continuous intragastric administration for 15 days according to the dosage.After the last administration for 1 h,APAP was injected intraperitoneally to establish liver injury model in each group except CON group.The serum levels of AST and ALT of mice were determined,and the levels of MDA,SOD,GSH,and GSH-PX in the liver of mice were tested.HE staining of the livers was performed to observe pathological changes.The expressions of Nrf2,Keapl and HO-1 proteins in the livers were detected by Western Blot.Results:The optimal purification conditions were as follows:10 g of D101 resin,2.0 mg/mL of up-sampling solution,up-sampling volume of 30 mL,50 mL of 80％ethanol solution elution.After purification,the flavonoid content was 70.32％±1.49％.Compared with CON group,the expressions of AST and ALT in the serum were significantly higher in MOD group(P＜0.01),indicating successful modeling of liver injury.Compared with MOD group,the liver index,AST,ALT,MDA,GSH levels and Keap 1 protein expression in PF-given groups were significantly reduced by 25％～60％(P＜0.01 or P＜0.05),the concentrations of SOD,GSH-PX,and the expression levels of Nrf2 and HO-1 protein were significantly increased by 15％～35％(P＜0.01 or P＜0.05).HE staining results showed that PF could significantly improve APAP-induced liver injury.Conclusion:The PF can protect APAP-induced acute liver injury,and its mechanism is related to the Nrf2/HO-1 signaling pathway which regulates oxidative stress.关键词
紫苏/总黄酮/对乙酰氨基酚/肝损伤Key words
Perilla/flavonoids/acetaminophen/liver injury分类
轻工纺织引用本文复制引用
孟庆霖,褚齐,宋健,董红畅,李贺,林珈羽,赵南晰..紫苏叶总黄酮对APAP诱导的急性肝损伤的保护作用[J].食品工业科技,2024,45(14):344-351,8.基金项目
吉林市科技创新发展计划项目(紫苏叶总黄酮对小鼠非酒精性肝损伤的保护作用及机制研究) （紫苏叶总黄酮对小鼠非酒精性肝损伤的保护作用及机制研究）
北华大学自然科学青年科研创新团队(BHQNTD202202). （BHQNTD202202）
