基于SSR标记的紫薇分子鉴定及遗传分析OACSTPCD

In this study,40 excellent samples were used as test materials,including'Dynamite'(G2),E1,E4,W9 and their hybrid offspring,14 pairs of SSR polymorphism primers between intraspecific parents were used to identify the offspring,and GeneMarker,Popgene,Cervus and NTSYS software were used to estimate genetic di-versity parameters,clustering analysis,AMOVA analysis and principal coordinate analysis of parents and their offspring.The results were as follows:(1)Thirty-eight samples were identified as true hybrids,with a hybridiza-tion rate of 95%.(2)The average polymorphism information(PIC)of the 14 primer pairs was 0.540 2,which was a high polymorphic level,the number of alleles(Na)was 4.5,the average expected heterozygosity(He)and Nei′ s gene genetic diversity index(H)were 2.602 2 and 0.594 7,respectively,and the average Shannon's information index was 1.107 9.(3)Hubei wild varieties of E1 were clustered in a branch,while E4,W9 and G2 from Ameri-can varieties were clustered on a large branch,the other 23 individuals were different from the three parents G2,E4 and W9,as a single branch.The 5 individuals of the M2 were clustered together with 24(M4)and 32(M5),these samples formed 3 large groups overall.Most of these individuals were clustered in the same branch as their parents,but some individuals were grouped into a single branch,which also indicated that some hybrid offspring had genetic variation,reflecting rich genetic diversity.(4)Both AMOVA analysis and principal coordinate analysis showed there was a wealth genetic variation within different samples.In summary,the SSR markers can effec-tively identify intraspecific hybrids and reflect the genetic diversity within the L.indica,which provides a scien-tific basis for the molecular identification of L.indica,and also lays a theoretical foundation for the later germplasm resource collection,the construction of core germplasm and the creation of new intraspecific varieties.