安徽农业大学学报2024，Vol.51Issue(3)：397-403,7.DOI:10.13610/j.cnki.1672-352x.20240710.017
基于SSR标记的紫薇分子鉴定及遗传分析
Molecular identification and genetic analysis of Lagerstroemia indica based on SSR markers
摘要
Abstract
In this study,40 excellent samples were used as test materials,including'Dynamite'(G2),E1,E4,W9 and their hybrid offspring,14 pairs of SSR polymorphism primers between intraspecific parents were used to identify the offspring,and GeneMarker,Popgene,Cervus and NTSYS software were used to estimate genetic di-versity parameters,clustering analysis,AMOVA analysis and principal coordinate analysis of parents and their offspring.The results were as follows:(1)Thirty-eight samples were identified as true hybrids,with a hybridiza-tion rate of 95%.(2)The average polymorphism information(PIC)of the 14 primer pairs was 0.540 2,which was a high polymorphic level,the number of alleles(Na)was 4.5,the average expected heterozygosity(He)and Nei′ s gene genetic diversity index(H)were 2.602 2 and 0.594 7,respectively,and the average Shannon's information index was 1.107 9.(3)Hubei wild varieties of E1 were clustered in a branch,while E4,W9 and G2 from Ameri-can varieties were clustered on a large branch,the other 23 individuals were different from the three parents G2,E4 and W9,as a single branch.The 5 individuals of the M2 were clustered together with 24(M4)and 32(M5),these samples formed 3 large groups overall.Most of these individuals were clustered in the same branch as their parents,but some individuals were grouped into a single branch,which also indicated that some hybrid offspring had genetic variation,reflecting rich genetic diversity.(4)Both AMOVA analysis and principal coordinate analysis showed there was a wealth genetic variation within different samples.In summary,the SSR markers can effec-tively identify intraspecific hybrids and reflect the genetic diversity within the L.indica,which provides a scien-tific basis for the molecular identification of L.indica,and also lays a theoretical foundation for the later germplasm resource collection,the construction of core germplasm and the creation of new intraspecific varieties.关键词
紫薇/SSR分子标记/真杂种鉴定/遗传多样性/种质资源Key words
Lagerstroemia indica/SSR molecular markers/identification of true hybrid/genetic diversity/germplasm resources分类
农业科技引用本文复制引用
杨姝琦,杨彦伶,彭婵,张新叶,李振芳..基于SSR标记的紫薇分子鉴定及遗传分析[J].安徽农业大学学报,2024,51(3):397-403,7.基金项目
湖北省自然科学基金(2018CFB703)资助. （2018CFB703）
