安徽医科大学学报2024，Vol.59Issue(5)：780-788,9.DOI:10.19405/j.cnki.issn1000-1492.2024.05.006
锁阳提取物对阿尔茨海默病模型小鼠认知功能障碍的影响研究
Study on the effects of extracts from cynomorium songaricum on cognitive dysfunction of Alzheimer's disease model mice
摘要
Abstract
Objective To investigate the effects of cynomorium songaricum extract on cognitive dysfunction of Alzheimer disease (AD) model mice based on network pharmacology and animal experiments.Methods Network pharmacology was used to predict the related targets and signal pathways of the extract of cynomorium songaricum to improve AD.Senescence accelerated mice P8 (SAMP8) were selected as the model of AD.Based on the results of the preliminary experiment, 0.17 g/(kg·d) was selected as the optimal dosage for the extract of cynomorium son-garicum.The extract of cynomorium songaricum [0.17 g/(kg·d) , Donepezil hydrochloride [2.0 mg/(kg·d) ] and normal saline were given orally for 28 days according to the groups.Morris water maze evaluated the learning and cognitive function of animals.The number of neurons in cornu ammonis 1 (CA1) of hippocampus was observed by Nissl staining.The expression of recombinant Beclin 1(Beclin-1), Sequestosome 1 (p62), light chain 3 (LC-3) protein was detected by immunohistochemical method.The protein expression levels of phosphoinositide 3-ki-nase (PI3K), protein kinase B (AKT) and glycogen synthase kinase3β(GSK-3β) in the hippocampus of mice in each group were detected by Western blot.Results Based on the network pharmacology study, it was predicted that the biological mechanism of cynomorium songaricum to improve AD might be the regulation of autophagy, and the possible signaling pathway was PI3 K/AKT/GSK-3β.The results of animal experiments showed that the extract of cynomorium songaricum could improve the spatial memory learning ability of AD model mice, improve the dam-age of hippocampal neurons, significantly increase the number of neurons, and increase the expression levels of PI3K, p-AKT/AKT, p-GSK-3β/GSK-3β, Beclin-1 and LC3 in the hippocampus of mice.The expression level of p62 decreased.There was no significant difference between male and female mice during the experiment.Conclu-sion The extract may improve the cognitive dysfunction of male and female AD models by activating autophagy mediated by PI3K-AKT-GSK-3β signaling pathway, and there is no significant gender difference in the effect.关键词
锁阳/阿尔茨海默病/网络药理学/自噬/PI3K-AKT-GSK-3β信号通路Key words
cynomorium songaricum/Alzheimer disease/network pharmacology/autophagy/PI3K-AKT-GSK-3β signaling pathway分类
医药卫生引用本文复制引用
柴晓盈,任琪,张剑平,吴丽娥,贾建新..锁阳提取物对阿尔茨海默病模型小鼠认知功能障碍的影响研究[J].安徽医科大学学报,2024,59(5):780-788,9.基金项目
国家自然科学基金(编号:81860215) （编号:81860215）
内蒙古自治区高等学校"青年科技英才支持计划"(编号:NJYT-20-A08) （编号:NJYT-20-A08）
