腹部推拿对UC模型大鼠背根神经节PLC/IP3R/Ca2+信号通路及AKT、p-AKT蛋白表达的影响OA

Objective:To observe the effect of abdominal massage on PLC/IP3R/Ca2+signaling pathway and the expression of AKT and p-AKT pro-teins in dorsal root ganglia of ulcerative colitis(UC)model rats,so as to explore the mechanism of abdominal massage in treating visceral hypersensi-tivity of ulcerative colitis.Methods:36 SPF rats were divided into 4 groups:blank group,model group,massage group,and mesalazine group.Ex-cept for the rats of blank group,the rats were prepared by drinking 5％dextran sulfate sodium salt(DSS)freely to establish UC model.From the sec-ond day of modeling,the rats of massage group were intervened with abdominal massage,the rats of mesalazine group were given mesalazine solu-tion by gavage,and the blank group and model group were only restrained and fixed in the fixator in the prone position.After 15 days of intervention,the calcium concentration of dorsal root ganglion(DRG)cells was detected by o-cresolphthalein complexed copper colorimetric method.The phos-pholipase C(PLC),inositol 1,4,5-triphate(IP3)and inositol 1,4,5-triphate receptor(IP3R)of DRG tissues were detected by ELISA.The protein ki-nase B(AKT)and phosphorylated protein kinase B(p-AKT)of DRG tissues were detected by WestemBlot.Results:Compared with the model group,the concentration of Ca2+,IP3 and IP3R in the massage group showed decreased,among which the concentration of Ca2+was significantly dif-ferent(P＜0.01),and there was no difference in other indexes(P＞0.05).The content of PLC and the expression of AKT and p-AKT proteins in the massage group showed increased and there was no difference(P＞0.05).Conclusion:The mechanism of abdominal massage may be related to the in-hibition of PLC-IP3R-Ca2+signaling pathway and the promotion of AKT activation in dorsal root ganglia of UC rats.