柱花草SgMPK6互作蛋白的筛选与验证OA北大核心CSTPCD

The mitogen-activated protein kinase (MAPK) cascade plays a key role in regulating plant immunity. Anthracnose is a serious disease affecting the production of Stylosanthes (Stylo). The SgMPK6 gene of Stylo has been shown to function in resistance against anthracnose,which is caused by Colletotrichum gloeosporioides. To investigate the interacting proteins downstream of SgMPK6 in the response to C. gloeosporioides infection,a yeast two-hybridization technique was used to screen the cDNA library of Stylo using the SgMPK6 kinase domain as the bait. Seventy-four potential interacting proteins were obtained. The interactions between the SgMPK6 kinase domain and the bHLH transcription factors SgbHLH32,SgbHLH33,and SgbHLH44 were verified in yeast two-hybrid experiments. Phosphorylation site analysis revealed that all three transcription factors contained MAPK phosphorylation sites. The results of qRT-PCR analyses showed that SgbHLH32,SgbHLH33,and SgbHLH44 were significantly up-regulated in Stylo by C. gloeosporioides infection. Together,these results suggest that SgbHLH32,SgbHLH33,and SgbHLH44 are substrates of SgMPK6 and may be involved in resistance against anthracnose. The results of this study provide a basis for further analysis of the role of SgMPK6 in resistance to C. gloeosporioides infection.