南方医科大学学报2024，Vol.44Issue(7)：1382-1388,7.DOI:10.12122/j.issn.1673-4254.2024.07.18
地五养肝方对非酒精性脂肪肝小鼠循环外泌体溶血甘油磷脂的调控作用
Regulatory effect of Diwu Yanggan Decoction on lysoglycerophospholipids in circulating exosomes in a mouse model of nonalcoholic fatty liver disease
摘要
Abstract
Objective To evaluate the regulatory effect of Diwu Yanggan(DWYG)Decoction on lysoglycerophospholipids(Lyso-GPLs)in circulating exosomes in a mouse model of nonalcoholic fatty liver disease(NAFLD).Methods Circulating exosomes isolated from mouse serum by size exclusion chromatography were morphologically characterized using transmission electron microscope and examined for surface markers CD9,CD63 and TSG101 using Western blotting.Twenty-four male Kunming mice were randomized into 3 groups for normal feeding(control,n=8)or high-fat diet feeding for 1 week to induce NAFLD,after which the latter mice were given DWYG decoction(treatment group,n=8)or normal saline(model group,n=8)by gavage for 4 weeks.After the last treatment,blood samples were collected from the mice for testing serum TC,HDL-C,LDL-C,ALT and AST levels and isolating circulating exosomes.Using multivariate statistical analysis based on targeted metabolomics strategy,the potential biomarkers for Lyso-GPLs in the exosomes were screened.Results The isolated exosomes about 100 nm in size had a typical saucer-like structure with distinct double-layer membranes and a mean particle size of 137.5 nm and expressed the specific surface marker proteins CD9,CD63 and TSG101.The mouse models of NAFLD had significantly increased serum levels of TC,HDL-C,LDL-C and AST and lowered serum ALT level.A total of 43 Lyso-GPLs with significant reduction after DWYG Decoction treatment were identified in NAFLD mice.Conclusion DWYG Decoction can regulate Lyso-GPLs in circulating exosomes in NAFLD mice,which provides a new clue for studying the therapeutic mechanism of DWYG Decoction for liver disease.关键词
地五养肝方/循环外泌体/液质联用/溶血甘油磷脂/非酒精性脂肪肝Key words
Diwu Yanggan Decoction/circulating exosomes/UPLC-QTOF-MS/MS/lysoglycerophospholipid/nonalcoholic fatty liver disease引用本文复制引用
陈光亚,向星亮,曾兆祥,黄荣增,金姝娜,肖明中,宋成武..地五养肝方对非酒精性脂肪肝小鼠循环外泌体溶血甘油磷脂的调控作用[J].南方医科大学学报,2024,44(7):1382-1388,7.基金项目
湖北省中医药管理局中医药青年人才项目(ZY2023Q040) （ZY2023Q040）
