分析化学2024，Vol.52Issue(6)：778-788,11.DOI:10.19756/j.issn.0253-3820.231320
磁性微球在循环肿瘤细胞和病毒以及核酸分离富集和检测分析中的应用进展
Progress on Application of Magnetic Particles in Biomedical Assays Including Detection of Circulating Tumor Cell and Virus,Extraction and Detection of Nucleic Acids
摘要
Abstract
Magnetic beads (MB),composed of magnetic nanoparticles and biocompatible materials including cellulose,chitosan,silica and polystyrene,are ideal platforms for specific detection of cancer cells,virus and nucleic acids when loaded with antibodies,fluorescent and conjugation molecules. With the ease of separations using magnets,magnetic particles show tremendous advantages over other platforms and thus arising potentials in circulating tumor cell (CTC),virus (such as SARS-CoV-2) antibody and antigen detections,and nucleic acid extractions and purifications. In addition,when combined with microfluidic devices,magnetic particles can be used to develop detection platforms with high throughputs,as well as portable and point-of-care apparatus. In accompany with their huge potentials in all kinds of biomedical and clinical scenarios,magnetic particles based medical devices have been commercialized such as antigen test kits that have been commonly used during the pandemic. In this review,the progress in detailed antibody conjugation practice used in each application scenario based on MB platform was summarized and the commercialization of analytical devices combined with microfluidics and others was discussed.关键词
磁性微球/循环肿瘤细胞/病毒抗原/核酸检测/评述Key words
Magnetic particles/Circulating tumor cell/Virus antigen/Nucleic acids detection/Review引用本文复制引用
郭玲香,周健平,杨涛..磁性微球在循环肿瘤细胞和病毒以及核酸分离富集和检测分析中的应用进展[J].分析化学,2024,52(6):778-788,11.基金项目
江苏省自然科学基金项目(No.BK20220801)资助.Supported by the Jiangsu Provincial Natural Science Foundation(No.BK20220801). （No.BK20220801）
