家畜生态学报2024，Vol.45Issue(7)：9-14,6.DOI:10.3969/j.issn.1673-1182.2024.07.002
14个山羊群体FGF5座位c.-253G＞A和507 bp缺失的遗传分析
Genetic Analysis of FGF5 Locus c.-253G＞A and 507 bp Deletion for 14 Goat Populations
摘要
Abstract
To identify the genetic markers related to cashmere traits and provide theoretical references for marker-assisted selection in goats,in this study,608 goats from 14 populations were genotyped using PCR-RFLP technology to detect allele frequencies at the c.-253G＞A and 507 bp deletion of the FGF5 lo-cus and analyze the correlation between the allele frequency and altitude and annual average temperature.Results showed the frequencies of the A allele at the c.-253G＞A and the Mut allele at the 507 bp deletion sites were 60.9％～98.3％and 95.7％～100％in five cashmere goat populations and three populations of Tibetan goats with dual-purpose for meat and cashmere,respectively.In six low-altitude populations,the frequencies of the A and Mut alleles varied from 0 to 51.3％and from 0.7％to 47.5％.The results also showed that excepting for the Qamdo Black Goat population,all 13 goat populations were in Hardy-Wein-berg equilibrium at both loci.The mutant allele frequency of the two loci was significantly correlated with altitude and annual average temperature(P＜0.001).These showed that the mutant allele frequencies at both c.-253G＞A and 507 bp deletion of FGF5 locus were extremely high in cashmere goats living in the areas with high altitude and relatively low temperatures.Therefore,both loci are the important sites af-fecting the cashmere length and can be used as molecular markers for genetic improvements of cashmere goats.关键词
绒山羊/绒毛长度/FGF5座位/多态性Key words
cashmere goat/fiber length/FGF5 locus/polymorphism分类
农业科技引用本文复制引用
向秋楠,黄清思,毛阿依,王小龙,宋天增,李利,张红平,郭家中..14个山羊群体FGF5座位c.-253G＞A和507 bp缺失的遗传分析[J].家畜生态学报,2024,45(7):9-14,6.基金项目
四川省自然科学基金(2023NSFSC0232) （2023NSFSC0232）
