14个山羊群体FGF5座位c.-253G＞A和507 bp缺失的遗传分析OA北大核心CSTPCD

To identify the genetic markers related to cashmere traits and provide theoretical references for marker-assisted selection in goats,in this study,608 goats from 14 populations were genotyped using PCR-RFLP technology to detect allele frequencies at the c.-253G＞A and 507 bp deletion of the FGF5 lo-cus and analyze the correlation between the allele frequency and altitude and annual average temperature.Results showed the frequencies of the A allele at the c.-253G＞A and the Mut allele at the 507 bp deletion sites were 60.9％～98.3％and 95.7％～100％in five cashmere goat populations and three populations of Tibetan goats with dual-purpose for meat and cashmere,respectively.In six low-altitude populations,the frequencies of the A and Mut alleles varied from 0 to 51.3％and from 0.7％to 47.5％.The results also showed that excepting for the Qamdo Black Goat population,all 13 goat populations were in Hardy-Wein-berg equilibrium at both loci.The mutant allele frequency of the two loci was significantly correlated with altitude and annual average temperature(P＜0.001).These showed that the mutant allele frequencies at both c.-253G＞A and 507 bp deletion of FGF5 locus were extremely high in cashmere goats living in the areas with high altitude and relatively low temperatures.Therefore,both loci are the important sites af-fecting the cashmere length and can be used as molecular markers for genetic improvements of cashmere goats.