玉米烯酮与氟苯尼考对雏鸡免疫功能和生长性能的影响OA北大核心CSTPCD

Zearalenone(ZEN)is a common environmental pollutant,while florfenicol(FFC)is a very widely used antimicrobial drug in veterinary clinic.They are highly likely to be exposed at the same time and have toxic and side effects on animals,but no relevant studies have been reported.To investigate the effects of single and combined exposure to ZEN and FFC on growth performance and immune function of chicks,forty 1-day-old chicks were adaptively fed for 2 days,then they were randomly divided into 4 groups with 10 chicks(half male and half female)in each group.The chicks in Control group were fed with a basal diet,and those in ZEN group were poisoned by gavage with dose of 1 mg·kg-1 diet for 21 days,those in FFC group were treated by water administration with 100 mg·L-1 drinking water for the first 5 days,while those in ZEN+FFC group were poisoned by gavage with dose of 1 mg·kg-1 diet for 21 days and treated by water administration with 100 mg·L-1 drinking water for the first 5 days.The experiment lasted 21 days.The average daily gain,organ index and feed to gain ratio of chicks were measured.Serum levels of cytokines and Newcastle disease antibody were detected by ELISA kit.The results showed that al-though ZEN was at low pollution level,it could inhibit the secretion of IL-2 and IFN-γ,thereby inhibiting immune function of chicks,and might induce inflammatory response by promoting the release of IL-1,IL-6 and TNF-α.Early treatment of FFC could not only inhibit the growth of chicks,but also down-regulate the secretion of IL-2 and IFN-γ,resulting in immunosuppression.The combined exposure to ZEN and FFC mitigated their inhibition to IL-2 and IFN-γ and controlled inflammatory responses induced by ZEN,how-ever,it also inhibited the humoral immune response of chicks.Therefore,the monitoring of ZEN contami-nation in the feed of chicks should be enhanced.FFC should be cautiously used for health protection drug-sin chicks since they were born.In addition,vaccination should be avoided when chicks were exposed to both ZEN and FFC in order to prevent the failure of immunization.