家畜生态学报2024，Vol.45Issue(7)：35-40,6.DOI:10.3969/j.issn.1673-1182.2024.07.006
发酵黄芪对肉鸡小肠黏膜上皮杯状细胞数量及MUC2基因表达的影响
Effects of Fermented Astragalus on the Number of Epithelial Goblet Cells and Expression of MUC2 Gene in Small Intestinal Mucosa of Broilers
摘要
Abstract
In order to study the effects of fermentation astragalus on the number of goblet cells and the expression of MUC2 gene in small intestinal mucosa of broilers,60 healthy one-day-old broilers with simi-lar initial body weight were selected and randomly divided into 4 groups,namely,unfermented astragalus group,fermented astragalus group,lactobacillus group and blank control group,respectively.The experi-ment lasted for 42 days,and samples were collected at 21 and 42 days of age.The number of goblet cell was counted by paraffin section-PAS staining and the expression of MUC2 gene was detected using RT-PCR,and the differences among the experimental groups was analyzed.The results showed compared with the control group,the number of goblet cells in duodenum,jejunum and ileum mucosa of 21-day-old broil-ers in fermented astragalus group increased by 61.30％,77.63％and 61.75％respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 67.89％,64.35％and 67.14％respectively(P＜0.01);the number of goblet cells in duodenum,jejunum and ileum mu-cosa of 42-day-old broilers in fermented astragalus group increased by 70.89％,56.38％,40.18％(P＜0.01)respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 58.21％,54.61％,56.06％respectively(P＜0.01).And those in unfermented astrag-alus group also increased(P＜0.05),with a slight difference in lactobacillus group.The results indicated that fermented astragalus could increase the number of intraepithelial goblet cells and the relative expres-sion of MUC2 gene in all segments of small intestinal mucosa,and the effect is better than lactobacillus and unfermented astragalus.关键词
发酵黄芪/肠黏膜免疫/杯状细胞/MUC2Key words
fermented astragalus/intestinal mucosal immune/goblet cell/MUC2分类
农业科技引用本文复制引用
李月勤,宋予震,史洪涛,王军,张利卫,彭永帅,乔宏兴,栗晓,杨树..发酵黄芪对肉鸡小肠黏膜上皮杯状细胞数量及MUC2基因表达的影响[J].家畜生态学报,2024,45(7):35-40,6.基金项目
河南省高等学校青年骨干教师培养计划项目(2018GGJS164) （2018GGJS164）
河南省科技攻关项目(222102110334) （222102110334）
河南省高等学校教学名师项目(教高[2020]434号) （教高[2020]434号）
河南省高等学校重点科研项目(22B230004) （22B230004）
