发酵黄芪对肉鸡小肠黏膜上皮杯状细胞数量及MUC2基因表达的影响OA北大核心CSTPCD

In order to study the effects of fermentation astragalus on the number of goblet cells and the expression of MUC2 gene in small intestinal mucosa of broilers,60 healthy one-day-old broilers with simi-lar initial body weight were selected and randomly divided into 4 groups,namely,unfermented astragalus group,fermented astragalus group,lactobacillus group and blank control group,respectively.The experi-ment lasted for 42 days,and samples were collected at 21 and 42 days of age.The number of goblet cell was counted by paraffin section-PAS staining and the expression of MUC2 gene was detected using RT-PCR,and the differences among the experimental groups was analyzed.The results showed compared with the control group,the number of goblet cells in duodenum,jejunum and ileum mucosa of 21-day-old broil-ers in fermented astragalus group increased by 61.30％,77.63％and 61.75％respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 67.89％,64.35％and 67.14％respectively(P＜0.01);the number of goblet cells in duodenum,jejunum and ileum mu-cosa of 42-day-old broilers in fermented astragalus group increased by 70.89％,56.38％,40.18％(P＜0.01)respectively,and the relative expression of MUC2 gene in each segment of small intestinal mucosa of broilers increased by 58.21％,54.61％,56.06％respectively(P＜0.01).And those in unfermented astrag-alus group also increased(P＜0.05),with a slight difference in lactobacillus group.The results indicated that fermented astragalus could increase the number of intraepithelial goblet cells and the relative expres-sion of MUC2 gene in all segments of small intestinal mucosa,and the effect is better than lactobacillus and unfermented astragalus.