江西农业大学学报2024，Vol.46Issue(4)：914-923,10.DOI:10.3724/aauj.2024081
桑椹菌核病菌的分离鉴定及室内药剂筛选
Isolation and identification of mulberry fruit sclerotiniosis pathogen and laboratory screening of biological fungicides
摘要
Abstract
[Objective]Mulberry fruit sclerotiniosis is a destructive disease on mulberry fruits,which poses a serious threat to the healthy development of the fruit and mulberry industry and yield stability.In order to provide the theoretical basis for the green prevention and control technology of mulberry fruit sclerotiniosis,the pathogens causing mulberry fruit sclerotiniosis in Le'an County,Jiangxi Province,were isolated and identified and the biopesticides with the ability of inhibiting the growth of the pathogens mycelium were screened.[Method]Tissue isolation method and Koch's rule were used to isolate and determine the pathogenicity of diseased mulberry in Le'an County,Jiangxi Province.Morphological and molecular biology methods were used to identify the isolated pathogens and optimize their cultivation conditions.The inhibitory effect of 7 biopesticides on the pathogen was measured using the drug containing plate method.[Result]Eight strains of fungi were isolated from the sclerotia collected from the planting site where mulberry fruit sclerotiniosis occurred,and cultured on PDA medium with consistent characteristics.The ITS and RPB2 sequences of representative strain JH21 were amplified and sequenced,and the strain JH21 was identified as Scleromitrula shiraiana by homology comparison.The mycelium of S.shiraiana grew better when it was incubated on mung bean dextrose agar medium(MBDA)at pH of 6 for 12 h with alternating light and dark and temperature conditions of 20-25℃.The laboratory screening of fungicides results showed that 0.3%Tetramycin aqueous solution and 0.3%Eugenol soluble solution had good inhibitory effects on the growth of S.shiraiana with EC50 of 0.078 μg/mL and 0.667 μg/mL respectively.[Conclusion]In this study,it was determined that mulberry plantations in Le'an County of Jiangxi Province were mainly threatened by reduced sorosis scleroteniosis caused by S.shiraiana.As biopesticides,Tetramycin and Eugenol are eco-friendly and safe,and they have potential applications in the prevention and control of mulberry fruit sclerotiniosis.关键词
桑椹菌核病/病原鉴定/核地杖菌/培养条件/生物农药Key words
mulberry fruit sclerotiniosis/pathogen identification/Scleromitrula shiraiana/cultivation conditions/biopesticides分类
农业科技引用本文复制引用
刘彤珂,赖家豪,熊桂红,游婧,蒋军喜,刘冰..桑椹菌核病菌的分离鉴定及室内药剂筛选[J].江西农业大学学报,2024,46(4):914-923,10.基金项目
国家自然科学基金项目(31460139)和江西省蚕桑产业技术体系(JXARS-23)Project supported by National Natural Science Foundation of China(31460139)and Jiangxi Sericultural Industry Technology System(JXARS-23) （31460139）
