中国临床保健杂志2024，Vol.27Issue(3)：346-350,5.DOI:10.3969/J.issn.1672-6790.2024.03.014
2型糖尿病患者血清E盒锌指蛋白1和泛素化特异性蛋白酶22表达水平与糖脂代谢及胰岛素抵抗的关系
Study on relationship between the expression of serum ZEB1 and USP22 and glucose lipid metabolism and insu-lin resistance in patients with type 2 diabetes
摘要
Abstract
Objective To detect the expression levels of serum zinc finger E-box-binding protein 1(ZEB1)and ubiquitin-specific protease 22(USP22)genes in patients with type 2 diabetes,and analyze the relationship between them and glucose and lipid metabolism and insulin resistance.Methods From June 2020 to June 2023,120 patients with type 2 diabetes diagnosed and treated in the First Affiliated Hospital of Suzhou University were collected as the study subjects(diabetes group).meantime,120 healthy people who underwent physical examination were as the control group.The qRT-PCR method was applied to detect the expression levels of ZEB1 mRNA and USP22 mRNA in serum;Pearson method was applied to analyze the correlation between the expression levels of ZEB1 mRNA and USP22 mRNA in serum of type 2 diabetes patients,and the correlation between them and BMI,TG,TC,FPG,FIns,HOMA-IR,ISI,HO-MA-β;multiple linear regression was applied to analyze the factors influencing the expression of ZEB1 mRNA and USP22 mRNA in serum of type 2 diabetes patients.Results The levels of BMI,TG,TC,FPG,FIns,HOMA-IR,ZEB1 mRNA and USP22 mRNA in the diabetes group were obviously higher than those in the control group,while ISI and HO-MA-β were obviously lower than those in the control group(P＜0.05).Pearson correlation analysis showed that there was a positive correlation between the expression of ZEB1 mRNA and USP22 mRNA in serum of type 2 diabetes patients(r=0.425,P＜0.001);the expression levels of serum ZEB1 mRNA and USP22 mRNA were positively correlated with FPG,FIns,HOMA-IR(P＜0.05),and negatively correlated with ISI and HOMA-β(P＜0.05).Multiple linear regres-sion analysis showed that an increase in FIns and a decrease in ISI were the influencing factors of serum ZEB1 mRNA expression level(P＜0.05);an increase in FPG was a factor influencing the expression level of USP22 mRNA(P＜0.05).Conclusions There is a positive correlation between the expression of ZEB1 mRAN and USP22 mRAN in the serum of type 2 diabetes patients,and the both are correlated with some indicators of glucose and lipid metabolism and insulin resistance.关键词
糖尿病,2型/E盒结合锌指蛋白1/泛素特异性蛋白酶类/代谢疾病/胰岛素抵抗Key words
Diabetes mellitus,type 2/Zinc finger E-box-binding homeobox 1/Ubiquitin-specific proteases/Met-abolic diseases/Insulin resistance引用本文复制引用
于冲,施毕旻,陆雯,廖蔼东,陆晓莲..2型糖尿病患者血清E盒锌指蛋白1和泛素化特异性蛋白酶22表达水平与糖脂代谢及胰岛素抵抗的关系[J].中国临床保健杂志,2024,27(3):346-350,5.基金项目
江苏省苏州市2023年度第十一批科技发展计划(基础研究-医学应用基础研究)项目(SKY2023006) （基础研究-医学应用基础研究）
