银杉愈伤组织诱导植株再生的影响因素OA北大核心CSTPCD
Factors Influencing the Callus Induction and Plant Regeneration of Cathaya argyrophylla
[目的]探索银杉成熟胚愈伤组织诱导植株再生的影响因素,为阐明影响诱导银杉愈伤组织、不定芽等的机制提供帮助.[方法]以广西金秀和广西桂林 2个种源的银杉成熟胚为试验材料,利用 1/2 MS、DCR、P6培养基,分别添加 0.5、1.0、1.5 mg·L-1 的 6-BA,0.2、0.4、0.6 mg·L-1 的NAA和 0.5、1.0、1.5 mg·L-1 的IBA,诱导银杉愈伤组织、不定芽和不定根,得到银杉再生植株;分析银杉种源、培养基、植物生长调节剂等对银杉愈伤组织、不定芽、不定根诱导率和增殖壮芽率的影响.[结果]广西桂林种源银杉愈伤组织、不定根诱导率和增殖壮芽率最大,分别为 66.48%,21.43%和 55.00%.广西金秀种源银杉不定芽诱导率最大,为 73.33%.适合银杉愈伤组织诱导植株再生的培养基和植物生长调节剂对于愈伤组织为DCR+0.5 mg·L-16-BA,对于不定芽为DCR+1.5 mg·L-16-BA,对于不定根为 1/2 MS+0.2 mg·L-1NAA+0.5 mg·L-1IBA,对于增殖壮芽为DCR+1.0 mg·L-16-BA+0.4 mg·L-1NAA.[结论]广西桂林种源银杉整体诱导率和增殖率相比广西金秀种源高.DCR培养基对银杉愈伤组织和不定芽诱导具有显著促进效果,1/2 MS培养基对不定根诱导具有显著促进效果.6-BA既可诱导银杉愈伤组织和又可诱导不定芽,NAA和IBA组合可诱导出银杉不定根,6-BA和NAA组合可对银杉不定芽进行增殖壮芽.
[Objective]Cathaya argyrophylla is a rare and endangered plant unique to China,however it is difficult to breed.In this study,the factors influencing the induction callus of plant regeneration from mature embryo of C.argyrophylla were studied,so as to provide assistance in further elucidating the mechanisms that influence the induction of callus and adventitious buds in C.argyrophylla.[Method]The mature embryos of C.argyrophylla from two provenances in Jinxiu and Guilin,Guangxi,were used as experimental materials.The media of 1/2 MS,DCR,and P6,with adding 0.5,1.0,and 1.5 mg·L-16-BA,0.2,0.4,and 0.6 mg·L-1NAA,and 0.5,1.0,1.5 mg·L-1IBA,respectively,were used to induce callus tissue,adventitious buds,and roots of C.argyrophylla,in order to obtain regenerated C.argyrophylla plants.The effects of two provenances,culture medium,plant growth regulators,etc.on the induction rate of callus,adventitious buds,adventitious roots,and proliferation rate of breeding and strengthening buds of C.argyrophylla were analyzed.[Result]The callus induction rate,adventitious root induction rate,and breeding and strengthening buds rate of C.argyrophylla from Guilin provenance were significantly higher,with values of 66.48%,21.43%,and 55.00%,respectively.The induction rate of adventitious buds in the C.argyrophylla from Jinxiu provenance was significantly higher,at 73.33%.The suitable culture medium and plant growth regulators for inducing plant regeneration from C.argyrophylla callus were:DCR+0.5 mg·L-16-BA for callus induction,DCR+1.5 mg·L-16-BA for adventitious bud induction,1/2 MS+0.2 mg·L-1NAA+0.5 mg·L-1IBA for adventitious root induction,and DCR+1.0 mg·L-16-BA+0.4 mg·L-1NAA for bud proliferation and strengthening.[Conclusion]The overall induction and proliferation rates of C.argyrophylla from Guilin,Guangxi are higher than those from Jinxiu,Guangxi.DCR medium has a significant promoting effect on the induction of callus and adventitious buds in C.argyrophylla,while 1/2 MS medium has a significant promoting effect on the induction of adventitious roots of C.argyrophylla.The 6-BA can induce both callus tissue and adventitious buds of C.argyrophylla.The combination of NAA and IBA can induce adventitious roots of C.argyrophylla.The combination of 6-BA and NAA can proliferate and strengthen adventitious buds of C.argyrophylla.
买凯乐;李荣珍;刘宏;覃琨;兰健花;朱昌叁;冯立新;苏杰南;周全连
广西生态工程职业技术学院 广西珍贵乡土树种良种培育中心 柳州 545004广西桂林花坪国家级自然保护区管理局 桂林 541700广西大瑶山国家级自然保护区管理局 金秀 545700广西壮族自治区林业科学研究院 南宁 530002
林学
银杉濒危物种成熟胚愈伤组织不定芽诱导植株再生
Cathaya argyrophyllaendangered speciesmature embryocallusadventitious bud inductionplant regeneration
《林业科学》 2024 (007)
56-64 / 9
广西青年基金项目(2017GXNSFBA198236);广西壮族自治区林业局林业科技项目(2024GXZCLK05).
评论