林业科学2024，Vol.60Issue(7)：56-64,9.DOI:10.11707/j.1001-7488.LYKX20230141
银杉愈伤组织诱导植株再生的影响因素
Factors Influencing the Callus Induction and Plant Regeneration of Cathaya argyrophylla
摘要
Abstract
[Objective]Cathaya argyrophylla is a rare and endangered plant unique to China,however it is difficult to breed.In this study,the factors influencing the induction callus of plant regeneration from mature embryo of C.argyrophylla were studied,so as to provide assistance in further elucidating the mechanisms that influence the induction of callus and adventitious buds in C.argyrophylla.[Method]The mature embryos of C.argyrophylla from two provenances in Jinxiu and Guilin,Guangxi,were used as experimental materials.The media of 1/2 MS,DCR,and P6,with adding 0.5,1.0,and 1.5 mg·L-16-BA,0.2,0.4,and 0.6 mg·L-1NAA,and 0.5,1.0,1.5 mg·L-1IBA,respectively,were used to induce callus tissue,adventitious buds,and roots of C.argyrophylla,in order to obtain regenerated C.argyrophylla plants.The effects of two provenances,culture medium,plant growth regulators,etc.on the induction rate of callus,adventitious buds,adventitious roots,and proliferation rate of breeding and strengthening buds of C.argyrophylla were analyzed.[Result]The callus induction rate,adventitious root induction rate,and breeding and strengthening buds rate of C.argyrophylla from Guilin provenance were significantly higher,with values of 66.48%,21.43%,and 55.00%,respectively.The induction rate of adventitious buds in the C.argyrophylla from Jinxiu provenance was significantly higher,at 73.33%.The suitable culture medium and plant growth regulators for inducing plant regeneration from C.argyrophylla callus were:DCR+0.5 mg·L-16-BA for callus induction,DCR+1.5 mg·L-16-BA for adventitious bud induction,1/2 MS+0.2 mg·L-1NAA+0.5 mg·L-1IBA for adventitious root induction,and DCR+1.0 mg·L-16-BA+0.4 mg·L-1NAA for bud proliferation and strengthening.[Conclusion]The overall induction and proliferation rates of C.argyrophylla from Guilin,Guangxi are higher than those from Jinxiu,Guangxi.DCR medium has a significant promoting effect on the induction of callus and adventitious buds in C.argyrophylla,while 1/2 MS medium has a significant promoting effect on the induction of adventitious roots of C.argyrophylla.The 6-BA can induce both callus tissue and adventitious buds of C.argyrophylla.The combination of NAA and IBA can induce adventitious roots of C.argyrophylla.The combination of 6-BA and NAA can proliferate and strengthen adventitious buds of C.argyrophylla.关键词
银杉/濒危物种/成熟胚/愈伤组织/不定芽诱导/植株再生Key words
Cathaya argyrophylla/endangered species/mature embryo/callus/adventitious bud induction/plant regeneration分类
农业科技引用本文复制引用
买凯乐,李荣珍,刘宏,覃琨,兰健花,朱昌叁,冯立新,苏杰南,周全连..银杉愈伤组织诱导植株再生的影响因素[J].林业科学,2024,60(7):56-64,9.基金项目
广西青年基金项目(2017GXNSFBA198236) （2017GXNSFBA198236）
广西壮族自治区林业局林业科技项目(2024GXZCLK05). （2024GXZCLK05）
