粮油食品科技2024，Vol.32Issue(4)：201-209,9.DOI:10.16210/j.cnki.1007-7561.2024.04.025
雨生红球藻中蛋白的结构和功能特性研究
Study on the Structure and Functional Characteristics of Proteins from Haematococcus pluvialis
摘要
Abstract
Haematococcus pluvialis residue powder,which has been extracted with astaxanthin,was used as the raw material to prepare Haematococcus pluvialis protein by alkaline extraction and acid precipitation method.The protein structure of Haematococcus pluvialis was characterized,and the effects of pH and ion strength on the functional characteristics of Haematococcus pluvialis protein were studied.The results showed that the isoelectric point of Haematococcus pluvialis protein was 4.2.The molecular weight of the subunit was around 14.4,18.4 and 38 kDa,respectively and the content of α-helix,β-fold,β-corner and disordered structure in the secondary structure was 14.86%,18.47%,40.3%and 26.37%,respectively.It contained 7 kinds of essential amino acids required by the human body,accounting for 36.38%of the total amino acid content.Microscopic observation showed that the protein of Haematococcus pluvialis was rod-shaped and sheet-like,with a rough and porous surface.With the increase of pH,the solubility,water absorption,emulsion capability,foam stability and foam stability of the protein of Haematococcus pluvialis increased first and then decreased,while the emulsification stability showed the opposite trend.With the increase of NaCl concentration,the foam capability,foam stability,emulsion capability and emulsification stability of the protein of Haematococcus pluvialis increased first and then decreased,while the solubility showed a trend of first decreasing,then increasing,and then decreasing again.关键词
雨生红球藻/蛋白质/制备/结构/功能特性Key words
Haematococcus pluvialis/protein/preparation/structure/functional characteristics分类
轻工纺织引用本文复制引用
李芹,王萌,袁朔,赵晓燕,刘红开,张晓伟,朱运平..雨生红球藻中蛋白的结构和功能特性研究[J].粮油食品科技,2024,32(4):201-209,9.基金项目
山东省自然科学基金面上项目(ZR2023MC198) （ZR2023MC198）
山东省重点研发计划(2019QYTPY058) Natural Science Foundation of General Project of Shandong Province(No.ZR2023MC198) （2019QYTPY058）
National Key Research and Development Project of Shandong Province(No.2019QYTPY058) （No.2019QYTPY058）
