南京林业大学学报（自然科学版）2024，Vol.48Issue(4)：57-66,10.DOI:10.12302/j.issn.1000-2006.202303009
氮磷配施对青钱柳生长及叶生物活性物质含量的影响
Effects of various ratios of nitrogen and phosphorus addition on the growth and contents of leaf bioactive substances in Cyclocarya paliurus
摘要
Abstract
[Objective]This study aimed to provide a theoretical basis for the strategic cultivation of plantations,and to increase the content of bioactive substances in leaves by investigating the effects of various nitrogen and phosphorus levels on the growth and bioactive substance content in Cyclocarya paliurus leaves.[Method]The research is conducted in the field and utilized a two-factor complete block design,incorporating three levels of nitrogen application(N0,N1,N2)and three levels of phosphorus application(P0,P1,P2),creating nine treatments with three replicates each.The growth indexes of seedlings and the contents of total flavonoids,total triterpenoids,total polyphenols and total polysaccharides in leaves were determined.A two-way ANOVA was used to compare the effects of nitrogen and phosphorus and their interaction on related indexes.[Result]The study found that different ratios of nitrogen and phosphorus significantly influenced both the growth and the bioactive substance content in C.paliurus leaves(P＜0.05).The contents of total flavonoids,triterpenoids,and polyphenols in the leaves exhibited a parabolic seasonal variation,peaking in July,while the highest polysaccharides content was observed in September.Compared to the control treatment(N0 P0),all nitrogen and phosphorus fertilizations enhanced tree growth,with the most substantial growth in tree height and ground diameter occurring in the N2 P2 treatment.The effects of nitrogen and phosphorus ratios on the bioactive substances varied,displaying differences among the substances and the sampling months sampled.The variation in the contents of total flavonoid,total triterpenoids,total polyphenols and total polysaccharides in June ranged from 17.01 to 34.25 mg/g,from 39.11 to 55.77 mg/g,from 22.74 to 53.98 mg/g,and from 22.36 to 33.24 mg/g,respectively,while in October,the ranges were 16.95-35.26,38.03-54.17,19.78-33.98 and 23.33-32.46 mg/g,respectively.Correlation analysis revealed no significant relationship between the monthly growth in tree height and the content of bioactive substances(total flavonoid,total triterpenoids,total polyphenols and total polysaccharides)in the leaves(P＞0.05).However,weakly negative correlations were observed between the ground diameter growth and the contents of total triterpenoids and total polyphenols in the leaves(P＜0.05).Notably,there were significant positive correlations between the growth of tree height and ground diameter,as well as among the different bioactive substances(P＜0.01).[Conclusion]A high ratio of nitrogen and phosphorus addition benefit the growth of C.paliurus and enhances the content of total triterpenoids in the leaves.Sole nitrogen or phosphorus supplements boost the content of secondary metabolites,whereas a combined application tends to decrease the content of total polysaccharides.There is a noticeable trade-off between the growth of C.paliurus and the content of secondary metabolites in its leaves.关键词
青钱柳/配方施肥/林木净生长量/生物活性物质Key words
Cyclocarya paliurus/fertilization/tree net growth/bioactive substances分类
农业科技引用本文复制引用
刘小芳,岳喜良,方升佐,李晴,孙昕..氮磷配施对青钱柳生长及叶生物活性物质含量的影响[J].南京林业大学学报（自然科学版）,2024,48(4):57-66,10.基金项目
国家自然科学基金项目(32071750) （32071750）
江苏省重点研发计划(现代农业)重点项目(BE2019388). （现代农业）
