南京农业大学学报2024，Vol.47Issue(4)：674-679,6.DOI:10.7685/jnau.202305029
桃拟茎点霉的侵染过程及其致病影响因子研究
Study on the infection process and pathogenic influencing factors of Phomopsis amygdali
摘要
Abstract
[Objectives]Peach shoot blight(PSB)greatly influenced the development of peach industry,Phomopsis amygdali which caused PSB was the most prevalent pathogens in peach orchard at south China.However,the infection process and pathogenic influencing factors remain unclear.Therefore,this study aimed to explore the infection process of P.amygdali and its pathogenic influencing factors.[Methods]In this work,conidial germination of P.amygdali and infection sites were observed through scanning electron microscopy(SEM).Different environmental conditions were used to explore pathogenic influencing factors of P.amgdali.Commercial fusicoccin was used to measured its virulence effect.[Results]The germination rate of α-conidia of P.amygdali was 66.4%at 3 hours post inoculation,97.6%at 9 h,then,single long germ tube produced at 24 h,septum formed at 30 h,and branching mycelium formed at 36 h.SEM observation showed that infective hyphae might mainly infect xylem and pith part of peach twigs,that destroyed the structure of xylem,and there were no infective hyphae at the phloem.Further,the study found that temperature and relative humidity(RH)were the main factors affecting the pathogenicity of P.amygdali.Among them,30℃and 98%RH were the best optimum temperature and relative humidity for the disease.Fusicoccin,one toxin produced by P.amygdali,was also one of pathogenic factors of P.amygdali.[Conclusions]In this work,the infection process of P.amygdali was investigated for the first time and the main environmental factors affecting its pathogenicity were temperature and RH.关键词
桃枝枯病/桃拟茎点霉/侵染过程/致病影响因子/致病力Key words
peach shoot blight/Phomopsis amygdali/infection process/pathogenic influencing factors/pathogenicity分类
农业科技引用本文复制引用
杨丽娜,郝靖璞,曹军,纪兆林..桃拟茎点霉的侵染过程及其致病影响因子研究[J].南京农业大学学报,2024,47(4):674-679,6.基金项目
国家自然科学青年基金项目(32202237) （32202237）
国家现代农业产业技术体系专项(CARS-30-3-02) （CARS-30-3-02）
