蝴蝶兰DREB基因家族鉴定及非生物胁迫表达分析OA北大核心CSTPCD

The dehydration responsive element binding(DREB)gene family plays an important role in plant response to abiotic stresses such as low temperature,drought,and high salinity,and currently,there are few reports on the DREB gene family in Phalaenopsis spp.In this study,Phalaenopsis aphrodite was used as the reference genome for the identification.In addition,the subcellular localization,bioinformatics,and gene expression pattern of DREB family members under 3 abiotic stress conditions:low temperature stress,drought treatment,and salt stress,and abscisic acid treatment were studied and analysed.Using conserved structural domain analysis and BLAST comparison,31 DREB family gene members were found from the P.aphrodite genome database.PaDREB transcription factors were divided into 6 subgroups,numbered A1 to A6,according to phylogenetic research.Conserved motif analysis revealed that all of PaDREB transcription factors had a AP2 conserved structural domain.The transcriptome expression pattern study of P.aphrodite revealed that different PaDREBs showed differential tissue expression and most of the genes were highly expressed in the roots.The 4 members of subgroup A1 were isolated and named PaDREB1A,PaDREB1B,PaDREB1C,and PaDREB1D,respectively,and subcellular localization was carried out on them,and the results showed that all of them were localized in the nucleus.Meanwhile,the results of expression analysis of the above 4 members showed that PaDREB1A mainly responded to drought and high salt and ABA treatments;PaDREB1B mainly responded to low temperature and high salt treatments;PaDREB1C mainly responded to drought and ABA treatments;and PaDREB1D mainly responded to low temperature,drought and ABA treatments.The above results suggested that the DREB family of Phalaenopsis had an important role in the response to abiotic stress pathway,and this study provides a theoretical basis for further research on the mechanism of the DREB family's response to abiotic stress in Phalaenopsis.