农业生物技术学报2024，Vol.32Issue(8)：1715-1728,14.DOI:10.3969/j.issn.1674-7968.2024.08.001
蝴蝶兰DREB基因家族鉴定及非生物胁迫表达分析
Identification of DREB Gene Family in Phalaenopsis aphrodite and its Expression Analysis Under Abiotic Stresses
摘要
Abstract
The dehydration responsive element binding(DREB)gene family plays an important role in plant response to abiotic stresses such as low temperature,drought,and high salinity,and currently,there are few reports on the DREB gene family in Phalaenopsis spp.In this study,Phalaenopsis aphrodite was used as the reference genome for the identification.In addition,the subcellular localization,bioinformatics,and gene expression pattern of DREB family members under 3 abiotic stress conditions:low temperature stress,drought treatment,and salt stress,and abscisic acid treatment were studied and analysed.Using conserved structural domain analysis and BLAST comparison,31 DREB family gene members were found from the P.aphrodite genome database.PaDREB transcription factors were divided into 6 subgroups,numbered A1 to A6,according to phylogenetic research.Conserved motif analysis revealed that all of PaDREB transcription factors had a AP2 conserved structural domain.The transcriptome expression pattern study of P.aphrodite revealed that different PaDREBs showed differential tissue expression and most of the genes were highly expressed in the roots.The 4 members of subgroup A1 were isolated and named PaDREB1A,PaDREB1B,PaDREB1C,and PaDREB1D,respectively,and subcellular localization was carried out on them,and the results showed that all of them were localized in the nucleus.Meanwhile,the results of expression analysis of the above 4 members showed that PaDREB1A mainly responded to drought and high salt and ABA treatments;PaDREB1B mainly responded to low temperature and high salt treatments;PaDREB1C mainly responded to drought and ABA treatments;and PaDREB1D mainly responded to low temperature,drought and ABA treatments.The above results suggested that the DREB family of Phalaenopsis had an important role in the response to abiotic stress pathway,and this study provides a theoretical basis for further research on the mechanism of the DREB family's response to abiotic stress in Phalaenopsis.关键词
蝴蝶兰/脱水响应元件结合蛋白(DREB)转录因子/生物信息学分析/亚细胞定位/非生物胁迫Key words
Phalaenopsis/Dehydration responsive element binding(DREB)transcription factor/Bioinformatics analysis/Subcellular localization/Abiotic stresses分类
园艺学与植物营养学引用本文复制引用
胡子昂,罗平,辛静静,崔永一..蝴蝶兰DREB基因家族鉴定及非生物胁迫表达分析[J].农业生物技术学报,2024,32(8):1715-1728,14.基金项目
浙江省农业新品种重大专项花卉育种专项(2021C02071-5) （2021C02071-5）
浙江省"尖兵""领雁"研发攻关计划项目(2023C02028) （2023C02028）
