大葱MAX1基因克隆及其对高温胁迫的响应OA北大核心CSTPCD

More axillary growth 1(MAX1)is a key gene in the strigolactone synthesis pathway,which plays an important role in the tillering regulation of green onions(Allium fistulosum)and resistance to adversity stress.In this study,AfMAX1 gene(GenBank No.AfisC2G04224)was cloned from green onions,and bioinformatics analysis,subcellular localization,tissue expression patterns,and high-temperature stress response analysis were carried out.The results showed that the CDS of AfMAX1 was 1 545 bp long,encoded 514 amino acids,and had a relative molecular weight of 58.55 kD.AfMAX1 was a hydrophilic protein with no signal peptide and transmembrane domain.Protein sequence comparison analysis showed that MAX1 was conserved in multiple species.Subcellular localization results showed that AfMAX1 might be localized in the cytoplasm and nucleus of Nicotiana benthamiana.Expression pattern analysis showed that AfMAX1 had the highest expression level in the leaf sheath,followed by in the roots;The expression level of AfMAX1 significantly increased after 12 h of high temperature stress(P＜0.01).The above results indicate that AfMAX1 could respond to high temperature stress,which might help enhance plant tillering regulation and heat tolerance.This study provides basic data for in-depth exploration of the molecular mechanisms of green onion plant type regulation and abiotic stress resistance.