农业生物技术学报2024，Vol.32Issue(8)：1776-1783,8.DOI:10.3969/j.issn.1674-7968.2024.08.006
大葱MAX1基因克隆及其对高温胁迫的响应
MAX1 Gene Cloning and Its Response to High Temperature Stress in Green Onions(Allium fistulosum)
摘要
Abstract
More axillary growth 1(MAX1)is a key gene in the strigolactone synthesis pathway,which plays an important role in the tillering regulation of green onions(Allium fistulosum)and resistance to adversity stress.In this study,AfMAX1 gene(GenBank No.AfisC2G04224)was cloned from green onions,and bioinformatics analysis,subcellular localization,tissue expression patterns,and high-temperature stress response analysis were carried out.The results showed that the CDS of AfMAX1 was 1 545 bp long,encoded 514 amino acids,and had a relative molecular weight of 58.55 kD.AfMAX1 was a hydrophilic protein with no signal peptide and transmembrane domain.Protein sequence comparison analysis showed that MAX1 was conserved in multiple species.Subcellular localization results showed that AfMAX1 might be localized in the cytoplasm and nucleus of Nicotiana benthamiana.Expression pattern analysis showed that AfMAX1 had the highest expression level in the leaf sheath,followed by in the roots;The expression level of AfMAX1 significantly increased after 12 h of high temperature stress(P＜0.01).The above results indicate that AfMAX1 could respond to high temperature stress,which might help enhance plant tillering regulation and heat tolerance.This study provides basic data for in-depth exploration of the molecular mechanisms of green onion plant type regulation and abiotic stress resistance.关键词
大葱/MAX1(more axillary growth 1)/高温胁迫/表达分析/生物信息学分析Key words
Allium fistulosum/More axillary growth 1(MAX1)/High temperature stress/Expression analysis/Bioinformatics analysis分类
植物保护学引用本文复制引用
张宇辰,柏咪凤,徐欢欢,邢嘉怡,周旭阳,刘乐承,王永勤..大葱MAX1基因克隆及其对高温胁迫的响应[J].农业生物技术学报,2024,32(8):1776-1783,8.基金项目
国家自然科学基金(31972409) （31972409）
北京市农林科学院科技创新能力项目(KJCX20200113) （KJCX20200113）
北京市农林科学院种质资源项目(KJCX20230126) （KJCX20230126）
