农业生物技术学报2024，Vol.32Issue(8)：1784-1791,8.DOI:10.3969/j.issn.1674-7968.2024.08.007
NtCPS2基因通过调控赤霉素合成影响烟草腺毛发生
NtCPS2 Gene Affects the Occurrence of Tobacco(Nicotiana tabacum)Glandular Hair by Regulating Gibberellin Synthesis
摘要
Abstract
The growth and development of foliar glandular hairs in tobacco(Nicotiana tabacum)is related to the aroma quality of tobacco leaves.In order to investigate the relationship between Ent-copalyl diphosphate synthase 2(NtCPS2)and the growth and development of glandular hair and the synthesis of glandular hair secretion in tobacco,the wild-type strain(WT),NtCPS2 homozygous editing strain(KD)and overexpression strain(OE)of'8306'were used as materials to observe the phenotype of glandular hair and the density of glandular hair.Content of cyperane substances was determined and the expression of key synthetic genes was analyzed.The results showed that compared with WT,the density of leaf secretory glandular hairs in KD strains increased by about 75%,and the secretion of leaf glandular hairs increased significantly(P＜0.05),while the secretion of leaf glandular hairs in OE strains was significantly lower than that of WT strains(P＜0.05),and decreased by about 50%compared with WT strains(P＜0.05).The expression level of cyclin B2(NtCycB2)gene in the negative regulation of glandular hair gene was significantly lower than that in WT and KD strain,and the expression level of NtCycB2 gene in OE and KD strains showed an opposite trend,and the expression level of NtCycB2 gene was opposite to that of glandular hair phenotype.The gibberellin content of KD tobacco plant was significantly higher than that of WT strain(P＜0.05).The gibberellin content of OE strains was significantly lower than that of WT strains(P＜0.05).The content of gibberellin in KD strains increased,the density of secretory glandular hairs increased,and the content of cyperane substances,the main glandular hair secretions in the foliar strain increased.The OE strain showed an opposite trend to the KD strain.The results suggested that the NtCPS2 gene might promote the increase of gonadal hair density and foliar glandular hair secretion by positively regulating gibberellin biosynthesis.This study provides a new research direction for the regulation of the synthesis of tobacco glandular hair and tobacco aroma compounds by NtCPS2gene through gibberellin pathway.关键词
烟草/叶面腺毛/柯巴基焦磷酸合酶(NtCPS2)/赤霉素/香气物质/代谢途径Key words
Tobacco/Foliar glandular hair/Ent-copalyl diphosphate synthase 2(NtCPS2)/Gibberellin/Aromatic substance/Metabolic pathway分类
农业科技引用本文复制引用
曹可心,杨惠娟,刘化冰,吴健,陈小翔,徐世晓..NtCPS2基因通过调控赤霉素合成影响烟草腺毛发生[J].农业生物技术学报,2024,32(8):1784-1791,8.基金项目
中国烟草总公司河南省公司重点项目(2021410000240021) （2021410000240021）
浙江中烟工业有限责任公司项目(ZJZY2021B009 （）
2023330000340127) （）
贵州省烟草公司铜仁市公司项目(202101) （202101）
