农业生物技术学报2024，Vol.32Issue(8)：1809-1820,12.DOI:10.3969/j.issn.1674-7968.2024.08.009
景宁木兰ZF-HD家族全基因组鉴定及其表达模式分析
Genome-wide Identification of ZF-HD Family and Its Expression Pattern Analysis in Magnolia sinostellata
摘要
Abstract
Stress from canopy shade is currently endangering a very small population of the indigenous Magnolia sinostellata found in Zhejiang province.A group of transcription factors known as zinc finger homeodomain(ZF-HD)is crucial for the plant development as well as the response to biotic and abiotic stress.In order to gain a better understanding of the mechanism underlying the MsZF-HD gene family under low-light stress,this study aimed to identify the members of the ZF-HD gene family and investigate their expression patterns based on transcriptome data.Twelve members of the MsZF-HD family were found using transcriptome data from M.sinostellata leaves and genomic data from M.biondii.The 12 MsZF-HDs located in the cell nucleus,and MsZF-HD2b also located in chloroplast.The ZF-HD_dimer structural domain was present in all members of the MsZF-HD gene family,which was revealed by phylogenetic analysis to be separated into 4 subfamilies.Additionally,the number and types of motifs were substantially conserved among the members of the same subfamily.According to GO analysis,members of the MsZF-HD family played a significant role in the expansion and evolution of M.sinostellata.Tissue-specific expression study revealed that most MsZF-HDs genes were substantially expressed in M.sinostellata leaf buds and leaves.MsZF-HD8 was highly expressed in both stems and leaves,while MsZF-HD13 and MsZF-HD14 were highly expressed in both flowers and stems,indicating that they might be involved in stem elongation and the regulation of floral development.Additionally,it was discovered that MsZF-HDs all responded favorably to low-light stress after studying the expression pattern of the MsZF-HDs gene under shade stress.Under low-light stress,the expression of MsZF-HD1a,MsZF-HD2b,MsZF-HD1b,MsZF-HD1c,MsZF-HD2a and MsZF-HD14 decreased.MsZF-HD13gene expression was significantly upregulated after 21 and 28 d of low-light treatment(P＜0.05).MsZF-HD11,MsZF-HD12 and MsZF-HD8 showed significant upregulation of gene expression after 7 d of low-light treatment(P＜0.05),followed by a downward trend.The ZF-HD family members of M.sinostellata were identified in this study and it was discovered that there was a significant expression response to low-light stress in leaf,stem and other tissues.MsZF-HD13 and MsZF-HD8 were found to be key genes in the response to shading to coordinate reproductive growth,stem elongation and leaf senescence.This study offers a theoreti-cal framework for examining M.sinostellata's shade response mechanism.关键词
锌指同源结构域(ZF-HD)转录因子家族/景宁木兰/弱光/基因表达Key words
Zinc finger homeodomain(ZF-HD)transcription factor family/Magnolia sinostellata/Low-light/Gene expression分类
农业科技引用本文复制引用
朱森宇,申亚梅,陈小珺,马晶晶,任明杰,王晶晶,吴超,田丰,刘志高,樊民亮..景宁木兰ZF-HD家族全基因组鉴定及其表达模式分析[J].农业生物技术学报,2024,32(8):1809-1820,12.基金项目
浙江省"十四五"育种专项花卉协作组项目(2021C02071-3) （2021C02071-3）
