冬凌草甲素对人软骨肉瘤SW-1353细胞的增殖和凋亡的影响及其机制探究OACSTPCD

Objective To study effects of oridonin on proliferation,apoptosis and p38 MAPK signaling pathway of human chondrosarcoma cells.Methods In vitro experiments,human chondrosarcoma SW-1353 cells were divided into control group(equal volume of DMSO solven),5,10,15 μmol/L oridonin group(5,10,15 μmol/L oridonin),10 μmol/L oridonin+inhibitor group(10 μmol/L oridonin+20 μmol/L p38 MAPK signaling inhibitor SB203580)and positive drug group(6 μmol/L doxorubicin).The inverted microscope,CCK-8,5-acetylene-2′ deoxyuracil riboside(EdU)staining,Hoechst 33258 staining,RT-qPCR and western blot assays were used to detect cell morphology,viability,proliferation,apoptosis and related fator levels.Results Compared with control group,cell viability,proliferation rate and levels of cyclin D1 and p-p38 MAPK were significantly decreased in the 5,10,15 μmol/L oridonin group,10 μmol/L oridonin+inhibitor group and positive drug group,while cell apoptosis and caspase-3 level were significantly increased(P<0.05).Compared with the10 μmol/L oridonin group,the addition of inhibitors resulted in more significant changes in each index.Conclusion Oridonin could significantly inhibit the proliferation and promote apoptosis of SW-1353 cells by inhibiting of p38 MAPK signal transduction.