针刺原俞配穴法对失眠大鼠脑组织p38MAPK信号通路的影响OACSTPCD

Objective:Based on p38 mitogen-activated protein kinase(p38MAPK)signaling pathway to investi-gate mechanism of acupuncture by Yuanshu acupoint macthing method on treatment of insomnia.Methods:50 rats were randomly divided into blank group,model group,original point group,back Shu point group and Yuanshu point matching group,with 10 rats in each group.Except for the blank group,four experimental groups were made by in-traperitoneal injection of chlorophenylalanine suspension(PCPA).After successful modeling,rats in the blank group and model group were only scratched and not acupuncture.The original point group were acupunctured on both sides of"Shenmen"and"Taichong".The back Shu point group were acupunctured on both sides of"Xinshu"and"Gan-shu".The Yuanshu point group were acupunctured on both sides of"Shenmen""Taichong","Xinshu","Ganshu".Af-ter the treatment,the general status of each group were observed,and expression of serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),p38MAPK protein and mRNA in hypothalamus were detected.Results:Compared with the blank group,rats in the model group showed significant weight loss,dark fur,messy and anxious,and serumIL-6(IL-6)and TNF-α(TNF-α)concentration and mRNA and protein expression levels of p38MAPK in the hypo-thalamus were significantly increased(P＜0.01).Compared to the model group,rats in the original point group,the back Shu point group and the Yuanshu point group had less weight loss,messy fur,significantly improved mental state,anxiety symptoms,serum IL-6(IL-6)and TNF-α(TNF-α)concentration and mRNA expression and protein ex-pression level of p38MAPK in the hypothalamus significantly decreased(P＜0.05).Effect of the Yuanshu point group was better than that in the Yuanshu group and the back Shu point group.Conclusion:Acupuncture can improve the sleep condition of rats with insomnia,and effect of the Yuanshu point acupuncture was better,which may work by in-hibiting p38MAPK signaling pathway and reducing inflammatory response.