兽类学报2024，Vol.44Issue(4)：411-426,16.DOI:10.16829/j.slxb.150867
食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响
Effects of methionine contents in food on the gut microbiota of plateau pika
摘要
Abstract
The degradation of grasslands caused by overgrazing provides abundant protein food resources for the plateau pika(Ochotona curzoniae)and changes in the content of essential amino acids(especially methionine)in the food can af-fect the host intestinal microbial community.In this study,plateau pikas were fed with 0.26％and 0.45％methionine di-ets according to the methionine content in their stomach contents in different degraded grasslands for 50 days.Collected feces were used for metagenomics and non-targeted metabolomics analysis to evaluate the effects of high methionine treatment on intestinal microbial communities and metabolites of plateau pika.The results showed that there was no sig-nificant difference in the α and β diversity of the intestinal microbial community of plateau pikas in the high methionine group compared with the low methionine group,but the abundance of Candidatus_Saccharibacteria and Tenericutes was significantly increased and the functions of butyric acid metabolism,taurine,and hypotaurine metabolism were signifi-cantly expressed,and the up-regulated metabolites were mainly enriched in primary bile acid synthesis,ascorbic acid and aldehyde metabolism,Th 17 cell differentiation,taurine and hypotaurine metabolism,B cell receptor signaling pathway,T cell receptor signaling pathway,and NF-κB pathway.On the other hand,the carbohydrate metabolism function of pla-teau pika microbiota in the low methionine group was significantly expressed,and its up-regulated metabolites were mainly enriched in amino acid metabolism-related pathways.These results suggest that high methionine foods can change intestinal microbes and their metabolites,which may enhance the immune and reproductive performance of the host,and play an important role in improving the individual fitness and population size of plateau pikas.关键词
高原鼠兔/蛋氨酸/肠道微生物/宏基因组学/非靶向代谢组学Key words
Plateau pika(Ochotona curzoniae)/Methionine/Gut microbes/Metagenomics/Non-targeted metabolomics分类
生物科学引用本文复制引用
董昕,陈慧青,张璐,尚国珍,吴雁,边疆晖..食物中蛋氨酸含量对高原鼠兔肠道微生物群落的影响[J].兽类学报,2024,44(4):411-426,16.基金项目
青海省自然科学基金青年项目(2021-ZJ-945Q) （2021-ZJ-945Q）
青海省人民政府中国科学院三江源国家公园联合专项(LHZX-2021-05) （LHZX-2021-05）
