高比例植物蛋白对青鱼幼鱼生长、消化、肝功能及血清生化指标的影响OA北大核心CSTPCD

The aim of this experiment was to study the effects of high proportion plant protein diet on the growth performance,digestive enzyme activity,liver function,and serum biochemical indices of Mylopharyngodon piceus.In the experiment,0,5％,10％,15％,and 20％fish meal protein were replaced by soybean meal(FM20,FM15,FM10,FM5,and SBM groups,respectively).A total of 800 Mylopharyngodon piceus were randomly divided into five groups with four replicates per group and 40 fish per replicate.The test period was 56 days.The results showed that the weight gain rate and specific growth rate in SBM group were significantly lower than those in FM20 group(P＜0.05),and the viscerosomatic index and hepatosomatic index were significantly lower than those in FM20 group,FM15 group,and FM10 group(P＜0.05).Hepatopancreas trypsin activity was the highest in SBM group,and enteric trypsin activity was the highest in FM20 group.The changes of trypsin and pepsin activity in different tissues were similar,and enteric amylase activity was higher than hepatopancreas,while lipase activity was lower than hepatopancreas.The activities of glutamic oxalic transaminase and glutamic pyruvic transaminase in serum and liver of SBM group were significantly higher than those in other groups(P＜0.05).The study shows that the use of appropriate plant protein in feed has no negative effects on the growth,digestion,liver function and biochemical indices of Mylopharyngodon piceus,and the minimum amount of fish meal is recommended to be 5％.