食品工业科技2024，Vol.45Issue(15)：1-9,9.DOI:10.13386/j.issn1002-0306.2023100066
超声辅助碱法提取无籽刺梨结合多酚工艺优化及其抗氧化活性分析
Optimization of Ultrasound-assisted Alkali Extraction of Bound Polyphenol from Rosa sterilis and Its Antioxidant Activity
摘要
Abstract
In order to investigate the application and development of Rosa sterilis in the field of drug-food homology,the Box-Behnken method was used to optimize the extraction process of bound polyphenols from Rosa sterilis based on the single factor experiment.The study also examined the clearance rate of DPPH·,ABTS+·,and·OH and total reducing power of bound polyphenols and free polyphenols from Rosa sterilis,with Vc as controls.The results showed that the optimal extraction conditions for the bound polyphenols of Rosa sterilis were a liquid-to-solid ratio of 24 mL/g,a lye concentration of 8 mol/L,an ultrasonic time of 47 min,and an ultrasonic power of 250 W.Under these conditions,the amount of extracted bound polyphenols was 135.73 mg GAE/g,which was close to the predicted value of the regression model(135.77 mg GAE/g).The model fit was excellent.The IC50 values of the bound polyphenols to DPPH and ABTS+.were 0.0599 and 0.0121 mg/mL,respectively.These values were significantly(P＜0.05)lower than those of the free polyphenols(0.168,0.0395 mg/mL).In the concentration range of 0.02～0.1 mg/mL,the scavenging ability of bound polyphenols on·OH was strong,which was not significantly different from that of VC(P＞0.05).The total reducing power of the bound polyphenols was higher than that of the free polyphenols.Additionally,the total reducing power of both polyphenols was significantly influenced by the concentration of the solution.The extracted bound polyphenols can be used as natural antioxidants,providing a theoretical basis for the processing and utilization of Rosa sterilis.关键词
无籽刺梨/结合多酚/抗氧化活性/超声辅助碱法Key words
Rosa sterilis/bound polyphenol/antioxidant activity/ultrasound-assisted alkali extraction分类
轻工纺织引用本文复制引用
秦毅臻,何洋,冯怡婷,范方宇..超声辅助碱法提取无籽刺梨结合多酚工艺优化及其抗氧化活性分析[J].食品工业科技,2024,45(15):1-9,9.基金项目
国家自然科学基金(31760470) （31760470）
云南省"兴滇英才支持计划"青年人才专项资助(YNWR-QNBJ-2018-046). （YNWR-QNBJ-2018-046）
