食品工业科技2024，Vol.45Issue(15)：34-44,11.DOI:10.13386/j.issn1002-0306.2023100246
大分子拥挤环境下多酚结构类似物抑制人血清白蛋白聚集的研究
Inhibition of Human Serum Albumin Aggregation by Polyphenols with Similar Structures in Macromolecular Crowding Environment
摘要
Abstract
To reveal the anti-protein aggregation ability of brazilin(Bra),hematoxylin(Hto)and hematein(Hte)under physiological crowding environment,the macromolecular crowding reagent(polyethylene glycol)was used to construct a simulated crowding environment.Then the inhibition mechanisms of these three structurally similar polyphenol compounds on the aggregation behavior of human serum albumin(HSA)were investigated by fluorescence spectroscopy,UV-vis absorption spectroscopy,dynamic light scattering(DLS)and atomic force(AFM)microscope assay.The results showed that they could maintain the stability of the HSA structure,decrease the number of amyloid fibrils and shorten the length of aggregates in crowding environment.Therefore,they inhibited the aggregation process of HSA,and the sequence of the inhibition ability was in the order of Hto＞Bra＞Hte.Moreover,the presence of crowding reagents could lead to a decrease in the inhibitory activity of these three inhibitors compared to in vitro dilute solution.In conclusion,this study suggested that Hto could be used as a potential protein aggregation inhibitor and a functional food ingredient for the treatment and intervention of amyloid-related diseases.关键词
巴西苏木素/苏木素/氧化苏木精/人血清白蛋白/蛋白质聚集/大分子拥挤Key words
brazilin/hematoxylin/hematein/human serum albumin/protein aggregation/macromolecular crowding分类
轻工纺织引用本文复制引用
姜梦瑶,闫雨,关姣,王梦凡,彭鑫..大分子拥挤环境下多酚结构类似物抑制人血清白蛋白聚集的研究[J].食品工业科技,2024,45(15):34-44,11.基金项目
国家自然科学基金青年项目(21506152). （21506152）
