食品工业科技2024，Vol.45Issue(15)：161-167,7.DOI:10.13386/j.issn1002-0306.2023080314
块菌贮藏期间细菌菌相分析及精油抑菌研究
Bacterial Flora and Essential Oils Antibacterial Activity during Storage of Tuber indicum
摘要
Abstract
In order to reveal the surface microbial changes of Tuber indicum during storage at 4 ℃,identify the dominant spoilage bacteria and select the natural antibacterial agent with good antibacterial effect.In this paper,high-throughput sequencing and traditional culture technology were used to study the surface flora structure and dominant spoilage bacteria of T.indicum during storage.Five natural plant essential oils were selected to study their inhibitory effects on dominant spoilage bacteria by measuring inhibitory rate and calculating their EC50 value.The results showed that the total number of colonies on the T.indicum surface was gradually increasing during storage,and reached 2.28×106 CFU/g on the 15th day which appeared odorous,sticky or moldy phenomena.Then reached the end of shelf life on the 30th day.Proteobacteria,Bacteroidetes,Actinobacteria,Firmicutes and Verrucomicrobia were the dominant phyla during storage.Pseudomonas、Rhizobium、Pedobacter、Sphingobacterium、Flavobacterium、Serratia、Janthinobacterium、Sphingopyxis and Variovorax were the dominant genera.Two strains of bacteria were isolated which could cause truffle decay rate of more than 90％,and identified as Bacillus subtilis and Brachybacterium faecium.Among the 5 essential oils,lemon grass essential oil had the best antibacterial effect on B.subtilis and the EC50 value was 5.471 μL/L,thyme essential oil had the best antibacterial effect on B.faecium and the EC50 value was 6.350 μL/L.It provides a theoretical basis for the further compound application of lemon grass and thyme essential oil in the postharvest storage and prolong shelflife of truffles.关键词
印度块菌/传统培养技术/高通量测序/优势腐败菌Key words
T.indicum/traditional culture technology/high-throughput sequencing/dominant spoilage bacteria分类
轻工纺织引用本文复制引用
清源,方志荣,吴瑕,姚昕,林巧,尹胜..块菌贮藏期间细菌菌相分析及精油抑菌研究[J].食品工业科技,2024,45(15):161-167,7.基金项目
四川省自然科学基金项目(2022NSFSC1712) （2022NSFSC1712）
攀西特色作物研究与利用四川省重点实验室项目(XNFZ2111). （XNFZ2111）
