食品工业科技2024，Vol.45Issue(15)：168-179,12.DOI:10.13386/j.issn1002-0306.2023070010
山茶各部位黄酮提取工艺优化及提取物成分分析
Extraction Optimization of Flavonoid Compounds from Various Parts of Camellia japonica and Constituent Analysis of the Extracts
摘要
Abstract
Objective:To optimize the extraction amount of flavonoid from petals,leaves,stamens and pistils of Camellia japonica,respectively,and compare the antioxidant activity and analyze the chemical constituents of the extracts.Method:The total flavonoids extraction amount was used as evaluation index,and single-factor experiments and orthogonal tests were used to optimize the ultrasonic-assisted enzymatic extraction process of flavonoids.The antioxidant capacity of optimized flavonoids extract was evaluated by DPPH and ABTS+free radical scavenging and reducing power experiment.And the chemical constituents of extracts from each part were qualitatively analyzed by ultra-performance-liquid-chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS).Results:In the optimum extraction condition,the total flavonoids extraction amount form each part of Camellia japonica were in the order as follows:Leaves(63.14 RE mg/g)＞stamens and pistils(58.77 RE mg/g)＞petals(20.26 RE mg/g).Meanwhile,the results of antioxidant activity determination showed that the IC50 values of DPPH and ABTS+free radical scavenging assay of extracts from petals,leaves,stamens and pistils were 7.28,2.51,1.15 mg/mL,and 2.85,0.95,0.59 mg/mL,respectively.And the reducing power value of extracts from petals,leaves,stamens and pistils were 63.25,214.11,475.90 Trolox mg/g.Therefore,the antioxidant activities of the extracts were in the order of stamens and pistils＞leaves＞petals.At last,a total of 29 flavonoid-related compounds were identified from the three parts by UPLC-QTOF-MS/MS,and the distribution pattern of B-type procyanidin dimer,epicatechin,quercetin-3-O-galactoside and some other flavonoid compounds in the three parts was consistent with that of total flavonoids extraction amount.Conclusion:Ultrasound-assisted enzymatic extraction effectively improved the extraction amount of flavonoids,and the leaves and stamens and pistils parts of Camellia japonica possessed higher flavonoid contents and stronger antioxidant activity.关键词
山茶/不同部位/黄酮类化合物/超声波辅助酶法/抗氧化活性/UPLC-QTOF-MS/MSKey words
Camellia japonica/different parts/flavonoids/ultrasound-assisted enzymatic extraction/antioxidant activity/UPLC-QTOF-MS/MS分类
轻工纺织引用本文复制引用
熊诗蓓,邓闯,范益飞,杨灵光..山茶各部位黄酮提取工艺优化及提取物成分分析[J].食品工业科技,2024,45(15):168-179,12.基金项目
江西省教育厅科学技术研究青年项目(GJJ211644,GJJ2201743) （GJJ211644,GJJ2201743）
宜春学院大学生创新创业项目(X202310417007,X202310417002). （X202310417007,X202310417002）
