食品工业科技2024，Vol.45Issue(15)：180-186,7.DOI:10.13386/j.issn1002-0306.2023070118
扁桃斑鸠菊叶促进灵芝胞外多糖高产条件优化及抗氧化活性研究
Optimization of High Yield Conditions and Antioxidant Activity of Ganoderma lingzhi Exopolysaccharide Promoted by Vernonia amygdalina Leaf
摘要
Abstract
Exopolysaccharide(EPS)is the main active metabolite of Ganoderma lingzhi in liquid fermentation.In order to increase the content of Ganoderma lingzhi EPS,this study added Vernonia amygdala leaf powder to the fermentation medium,and used single factor experiments and orthogonal experiments to optimize fermentation conditions.Fourier transform infrared spectroscopy was used to characterize the structure of Ganoderma lingzhi EPS,then its antioxidant activity was detected.The single factor experiment found that when the optimal addition amount of Vernonia amygdala leaf powder was 4g/L,the content of EPS increased by 167％compared with the control group.The optimum fermentation conditions of the orthogonal experiment were as follows:Fermentation duration was 12 d,initial pH was 5.0,the rotation speed was 120 r/min,and the addition amount of Vernonia amygdala leaf powder was 4 g/L.Under such a condition,the content of EPS could reach 13.05 g/L.The infrared spectra showed that the major absorption peaks of the Ganoderma lingzhi EPS from the addition of Vernonia amygdala leaf powder were similar to those of the EPS from the control group.The antioxidant activity assay showed that the addition of Vernonia amygdala leaf powder had less effect on the scavenging of ABTS+radicals by EPS,but enhanced their ability to scavenge DPPH radicals,OH radicals,and ferric ion reduction.The results showed that Vernonia amygdala leaf could effectively increase the content of EPS in Ganoderma lingzhi,which provided a new idea for the efficient production of polysaccharides in Ganoderma lingzhi.关键词
灵芝/胞外多糖/扁桃斑鸠菊叶/液体发酵/抗氧化活性Key words
Ganoderma lingzhi/exopolysaccharides/Vernonia amygdalina leaf/liquid fermentation/antioxidant activity分类
轻工纺织引用本文复制引用
汤坤鹏,刘盛荣,余明明,叶丽云,王圣铕,吴小平..扁桃斑鸠菊叶促进灵芝胞外多糖高产条件优化及抗氧化活性研究[J].食品工业科技,2024,45(15):180-186,7.基金项目
福建省特色优势食用菌工厂化专用品种及关键技术攻关(2022NZ029015). （2022NZ029015）
