食品工业科技2024，Vol.45Issue(15)：213-222,10.DOI:10.13386/j.issn1002-0306.2023080337
富硒羊肚菌多糖的制备及其体外抗肿瘤活性探究
Preparation of Selenium-enriched Morchella Polysaccharide and Its Antitumor Activity in Vitro
摘要
Abstract
This study aimed to optimize the extraction and purification processes for selenium-enriched Morchella esculenta(L.)Pers.(also known as Morchella)polysaccharides(Se-MPS)and to evaluate their anti-tumor activities and mechanisms.The extraction process was optimized using single-factor and response surface methodology.The crude polysaccharides were purified through column chromatography and their chemical composition was analyzed.The anti-tumor activity of Se-MPS was evaluated using HepG2 cells through a series of assays,including CCK-8,colony formation,scratching,and Transwell assays.The potential anti-tumor mechanisms of Se-MPS were assessed using RT-qPCR,Western blot,and immunofluorescence methods.Experimental results from single-factor and response surface methodology revealed that the optimal extraction conditions for selenium-enriched Morchella crude polysaccharides were:An extraction time of 2.5 h,a liquid-to-material ratio of 45:1(mL/g),and an extraction temperature of 82 ℃,yielding a crude polysaccharides yield of 7.95％.After purification,the total sugar content of Se-MPS reached 95.86％,with a selenium concentration of 76.07±0.32 mg/g.Furthermore,Se-MPS significantly reduced colony formation efficiency,cell migration rate,and the number of invasive cells in HepG2 cells(P＜0.05).Meanwhile,Se-MPS inhibited the PI3K/Akt signaling pathway,down-regulated mRNA expression of glucose transporter 2(GLUT2),and reduced glucose uptake in cells.The results indicated that optimizing extraction time,liquid-to-material ratio,and extraction temperature could improve the yield of selenium-enriched Morchella crude polysaccharides.Additionally,Se-MPS exhibited strong anti-tumor activity by regulating the PI3K/Akt pathway.This study provides evidence of the anti-tumor activities of Se-MPS and offers insights for the development of related functional foods.关键词
硒/羊肚菌多糖/化学组成/响应面优化/抗肿瘤活性Key words
selenium/Morchella polysaccharide/chemical composition/response surface optimization/anti-tumor activity分类
轻工纺织引用本文复制引用
李贝贝,王慧琴,孙仪征..富硒羊肚菌多糖的制备及其体外抗肿瘤活性探究[J].食品工业科技,2024,45(15):213-222,10.基金项目
河南省重点研发专项(221111112300) （221111112300）
郑州澍青医学高等专科学校骨干教师培养计划(2024ssgg04) （2024ssgg04）
郑州澍青医学高等专科学校2024年教师教学创新团队培养计划(2024jxcxtd01). （2024jxcxtd01）
