食品工业科技2024，Vol.45Issue(15)：233-243,11.DOI:10.13386/j.issn1002-0306.2023090036
白丁香花多糖的提取、纯化及抗氧化活性研究
Extraction,Purification and Antioxidant Activity of Polysaccharide from White Lilacs
摘要
Abstract
Enzyme-assisted ultrasonic extraction was employed to extract polysaccharides from white lilacs.The effects of various experimental conditions on the yield of polysaccharides from white lilacs were systematically analyzed using the response surface methodology(RSM).The crude extract was purified through DEAE-52 cellulose column and Sephadex G-100 gel column chromatography.High-performance gel permeation chromatography(HPGPC),ion chromatography(IC),ultraviolet spectrum analysis,Fourier infrared spectroscopy(FTIR)and scanning electron microscopy(SEM)were utilized for characterization of the polysaccharide structure and its antioxidant activity in vitro was investigated.The results demonstrated that the optimal extraction process for polysaccharides from white lilacs involved ultrasonic power 150 W,ultrasonic time 40 min,ultrasonic temperature 40 ℃,enzyme dosage 2.2％,solid-liquid ratio 1∶40 g/mL.Under these conditions,the yield of polysaccharides was 3.03％±0.09％.The polysaccharides were purified using a DEAE-52 cellulose column,and the main component SP-c was collected.Subsequently,SP-c-1 was obtained through a Sephadex G-100 gel column.The monosaccharide composition and molar ratio of SP-c-1 were galacturonic acid,arabinose,galactose,rhamnose,glucose,glucuronic acid,xylose=18.39∶11.13∶8.96∶2.61∶1∶0.83∶0.57,respectively.The weight-average molecular weight(Mw)of SP-c-1 was 14069 Da,and the numerical mean molecular weight(Mn)was 13637 Da.SP-c-1 had the characteristic absorption peak of polysaccharide and contained D-grape pyranose configuration.The half-inhibitory concentration(IC50)of SP-c-1 on DPPH and ABTS+free radicals was 0.87 and 1.355 mg/mL,respectively.Overall results indicate that SP-c-1 exhibits significant antioxidant activity.关键词
白丁香花/多糖/结构表征/酶辅助超声提取/抗氧化活性Key words
white lilacs/polysaccharide/structural characterization/enzyme-assisted ultrasonic extraction/antioxidant activity分类
轻工纺织引用本文复制引用
王天怡,张庆芬,黄磊磊,刘佳怡,杨逢建..白丁香花多糖的提取、纯化及抗氧化活性研究[J].食品工业科技,2024,45(15):233-243,11.基金项目
黑龙江省林业科技项目(Hljlykj2018-1). （Hljlykj2018-1）
