食品工业科技2024，Vol.45Issue(15)：295-303,9.DOI:10.13386/j.issn1002-0306.2023080195
一测多评法同时测定桑叶中9种绿原酸类和黄酮类成分
Simultaneous Determination of Nine Chlorogenic Acids and Flavonoids in Mori Folium by QAMS
摘要
Abstract
Objective:To establish a HPLC method for the determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutin,isoquercitrin,isochlorogenic acid B,astragalin,isochlorogenic acid A and isochlorogenic acid C in Mori Folium using the quantitative analysis of multi-components by single marker(QAMS).Methods:The analysis was performed on an Agilent TC-C18 column(250 mm×4.6 mm,5 μm),with acetonitrile(A)-0.1％phosphoric acid(B)as mobile phase at the flow rate of 1.0 mL/min for gradient elution,as well as the wavelengths were 260 and 320 nm and the column temperature was 30 ℃.The chlorogenic acid was used as the internal reference.The contents of nine components in Mori Folium were calculated by the external standard method and QAMS(multi-point correction,gradient correction and single-point correction)respectively,and the differences among the four methods were compared.In addition,the accuracy and feasibility of the method were verified by using multi-point correction and two-point correction to locate chromatographic peaks of the components.Results:The relative correction factors of neochlorogenic acid,cryptochlorogenic acid,rutin,isoquercitrin,isochlorogenic acid B,asiaticoside,isochlorogenic acid A and isochlorogenic acid C to chlorogenic acid were 0.9072,0.8736,0.6207,0.8547,1.1936,0.5501,1.4369 and 1.2244,respectively(multi-point correction).The durability(RSD＜1.5％)and the reproducibility(RSD＜5％)of the relative correction factors were positive under different conditions.The content of nine components in Mori Folium was determined simultaneously by the external standard method and QAMS(multi-point correction,gradient correction and single-point correction),and there was no difference between the results obtained from the four calculation methods(RSD＜1.5％).Compared to the relative retention time,multi-point correction and two-point correction could improve the accurate localization of the chromatographic peaks(relative error|RE|＜3％).Conclusion:The method of QAMS for nine components determination in Mori Folium was established,which was accurate and feasible and could be applied for the quality control of Mori Folium.关键词
高效液相色谱(HPLC)/一测多评/桑叶/绿原酸/斜率校正/单点校正/两点校正Key words
high performance liquid chromatography(HPLC)/QAMS/Mori Folium/chlorogenic acid/gradient correction/single-point correction/two-point correction分类
轻工纺织引用本文复制引用
刘梦文,沈静,阿依努尔·白克热,贺诗茹,肖辉,徐维盛..一测多评法同时测定桑叶中9种绿原酸类和黄酮类成分[J].食品工业科技,2024,45(15):295-303,9.基金项目
自治区创新环境(人才、基地)建设专项—科技创新基地建设计划(资源共享平台建设)(PT2018) （人才、基地）
中国营养学会全民营养科研基金(CNS-NNSRG2019-96). （CNS-NNSRG2019-96）
